Telomerase reverse transcriptase

Mục tiêu của đề tài là thiết kế thành công vector tái tổ hợp biểu hiện trong tế bào động vật pcDNA3.1(+)/hTERT mang đoạn gen mã hóa cho epitope của kháng nguyên telomerase reverse transcriptase (hTERT). Hệ vector tái tổ hợp này đƣợc bao gói trong nano chitosan.

73p closefriend03 13-10-2021 29 6   Download

Telomerase is a specialized reverse transcriptase responsible for synthesizing telomeric DNA at the ends of chromosomes. Six subunits composing the telomerase complex have been cloned: hTR (human telomerase RNA), TEP1 (telomerase-associated protein 1), hTERT (human telomerase reverse transcriptase), hsp90 (heat shock protein 90), p23, and dyskerin. In this study, we investigated the role of each the telomerase subunit on the activity of telomerase.

9p research12 01-06-2013 28 3   Download

To test the role of poly(ADP-ribose) polymerase on the telomerase activity, we determined the telomerase activity in leukemic cells K562 treated with benzamide and 4-amino 1,8 naphthalimide (NAP), the inhibitors of PARP. We observed that both the agents inhibited telomerase activity in a dose-dependent manner. The doses of benzamide and NAP that inhibited telo-merase activity to 50% of untreated control cells were 10.7 ± 0.6 mmand 200 ± 7lm, respectively.

12p fptmusic 11-04-2013 47 2   Download

Abstract To overcome loss of stem-like properties and spontaneous differentiation those hinder the expansion and application of human mesenchymal stem cells (hMSCs), we have clonally isolated permanent and stable human MSC lines by ectopic overexpression of primary cell cultures of hMSCs with HPV 16 E6E7 and human telomerase reverse transcriptase (hTERT) genes. These cells were found to have a differentiation potential far beyond the ordinary hMSCs. They expressed trophoectoderm and germline specific markers upon differentiation with BMP4 and retinoic acid, respectively.

13p toshiba23 18-11-2011 53 3   Download

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Immunohistochemical expression of h-telomerase reverse transcriptase in canine and feline meningiomas

5p hoami_238 17-09-2011 38 2   Download