The enzymatic hydrolysis

The results of microbiological and toxicological tests have proved the safety of the bone-muscular cod waste (BMW). Waste (humerus with meat cuts) from cod cutting on fillets contains 18,95 % of a full-grade animal protein and insignificant amount of fat (0.15 %).

14p lucastanguyen 01-06-2020 17 2   Download

We study the enzymatic hydrolysis of lactose by a commercial enzyme from a selected strain of Kluyveromyces fragilis. The variables analyzed were: temperature (25–40 ◦C), enzyme concentration (0.1–3.0 g l−1), lactose concentration (0.0278–0.208 M), and initial galactose concentration (0.0347 M). On the basis of the data analyzed, both published and in the present work, we propose a Michaelis–Menten kinetic model with inhibition by the product (galactose), which reveals that the substrate (lactose) and the product (galactose) present similar affinity for the active site of the enzyme.

10p abk1996 04-09-2018 33 1   Download

The purpose of this work was to study protein and lipid recovery from yellowfin tuna head by enzymatic hydrolysis as well as to analyse the amino acid compositions in tuna head protein hydrolysate and the fatty acid compositions in tuna head oil.

9p nganga_08 12-10-2015 81 3   Download

An exo-1,3-b-glucanase has been isolated from cultural filtrate of Trichoderma viride AZ36. The N-terminal sequence of the purified enzyme (m ¼ 61 ^ 1 kDa) showed no significant homology to other known glucanases. The 1,3-b-glucanase displayed high activity against laminarins, curdlan, and 1,3-b-oligoglucosides, but acted slowly on 1,3-1,4-b-oligoglucosides. No significant activity was detected against high molecular mass 1,3-1,4-bglucans. The enzyme carried out hydrolysis with inversion of the anomeric configuration.

9p system191 01-06-2013 44 6   Download

The carbohydrate moieties of glycosphingolipids from eggs of the human parasite,Schistosoma mansoni,wereenzy-matically released, labelled with 2-aminopyridine (PA), fractionated and analysed by linkage analysis, partial hydrolysis, enzymatic cleavage, matrix-assisted laser desorption/ionization time-of-¯ight mass spectrometry and nano-electrospray ionization mass spectrometry.

13p research12 23-04-2013 25 2   Download

The enzymatic properties of chitinase A fromVibrio carchariaehave been studied in detail by using combined HPLC and electrospray MS. This approach allowed the separation ofaandbanomers and the simultaneous monitoring of chitooligosaccharide products down to picomole levels. Chi-tinase A primarily generated b-anomeric products, indicating that it cata-lyzed hydrolysis through a retaining mechanism.

11p fptmusic 11-04-2013 30 2   Download

The enzymatic hydrolysis of cellulose encounters various limitations that are both substrate- and enzyme-related. Although the crystallinity of pure cellulosic Avicel plays a major role in determining the rate of hydrolysis by cellulases from Trichoderma reesei, we show that it stays constant during enzymatic conversion. The mode of action of cellulases was investigated by studying their kinetics on cellulose samples.

12p mobifone23 18-01-2013 48 5   Download

Problem statement: Dilute sulphuric acid and enzymatic hydrolysis methods were used for sugar extraction. Xylose and glucose sugars were obtained from corn cobs. Approach: Acid hydrolysis of corn cobs gave higher amount of sugars than enzymatic hydrolysis. Results: The results showed that optimal temperature and time for sugar fermentation were approximately 25°C and 50 h by two yeast strains (S. cerevisiae and P. Stipitis) respectively.

6p nguyenngocsonctu 30-11-2010 139 17   Download