The separation of proteins

Reconstructing phylogenetic trees from protein sequences normally requires empirical amino acid substitution models to calculate the likelihood of trees or genetic distances between species. The tree of life is classified into three domains of Eukaryotes, Archaea, and Bacteria. The amino acid substitution models have been intensively studied for decades, but few are related to Bacteria. Rooting bacterial trees remains a challenging problem in the phylogenetic analysis due to the long branch separating Bacteria and other domains.

14p dianmotminh02 03-05-2024 4 2   Download

Ebook "Microfluidic methods for molecular biology" covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis.

382p tracanhphuonghoa1007 22-04-2024 2 1   Download

Ebook "Advanced dairy chemistry - Volume 1B: Proteins: Applied aspects (Fourth edition)" covers the applied, technologically-focused chemical aspects of dairy proteins, the most commercially valuable constituents of milk. This fourth edition contains most chapters in the third edition on applied aspects of dairy proteins. The original chapter on production and utilization of functional milk proteins has been split into two new chapters focusing on casein- and whey-based ingredients separately by new authors.

508p tudohanhtau1006 29-03-2024 6 1   Download

Ebook "Proteomics in foods: Principles and applications" will discuss recent developments and the expected trends of the near future in food proteomics. The book will be divided into two parts. The first part (7 chapters) will focus on the basic principles for proteomics, e.g., sample preparation, such as extraction and separation techniques, analytical instrumentation currently in use, and available databases for peptide and protein identification.

589p coduathanh1122 27-03-2024 2 1   Download

Ebook "Field-flow fractionation in biopolymer analysis" collection of important biomedical applications for a set of separation/characterization techniques that are rapidly gaining popularity due to their wide dynamic range, high resolution, and ability to function in most commonly used solvent systems. Importantly, the field-flow fractionation (FFF) technique has recently emerged as a prominent complement to size exclusion chromatography for protein pharmaceuticals.

306p coduathanh1122 27-03-2024 9 1   Download

Part 1 book "Chromatography - The most versatile method of chemical analysis" by Leonardo de Azevedo Calderon includes contents: Purification of phospholipases A2 from American snake venoms; use of chromatography in animal ecology; 2D-NanoLC-ESI-MS/MS for separation and identification of mouse brain membrane proteins; analytical methods for quantification of drug metabolites in biological samples; current trends in sample treatment techniques for environmental and food analysis,... and other contents.

239p muasambanhan09 16-03-2024 4 1   Download

Ebook "Analytics of Protein–DNA interactions (Advances in biochemical engineering/biotechnology, Volume 104)" focuses on the fascinating possibilities that emerged during the last years to study protein–DNA interactions in vitro. As an essential part of transcriptional activity during development and in tumorgenesis a separate chapter describing the analysis of DNA modification especially methylation of cytosines at their carbon-5 position was included.

212p manmanthanhla0201 27-02-2024 4 2   Download

Ebook "Upscaling of bio-nano-processes: Selective bioseparation by magnetic particles" despite ongoing progress in nano- and biomaterial sciences, large scale bioprocessing of nanoparticles remains a great challenge, especially because of the difficulties in removing unwanted elements during processing in food, pharmaceutical and feed industry at production level. This book presents magnetic nanoparticles and a novel technology for the upscaling of protein separation.

250p nhanphanguyet 28-01-2024 3 2   Download

Part 1 book "Seafood processing" includes content: Introduction to seafood processing by products; trends in the use of seafood processing by products in Europe; prospective utilization of fishery by products in Indonesia; development of functional materials from seafood by products by membrane separation technology; processing and characterization of salt - fermented fish (jeotgal) using seafood by products in Korea; isoelectric solubilization precipitation as a means to recover protein and lipids from seafood by products,... and other contents.

317p muasambanhan01 13-12-2023 7 3   Download

Ebook "Structure and dynamics of confined polymers" provide insight on biological processes involving confinement and form a basis for new biotechnological applications using polymers. In his paper Edmund DiMarzio asks: What is so special about polymers? Why are polymers so prevalent in living things? The chemist says the reason is that a protein made of N amino acids can have any of 20 different kinds at each position along the chain, resulting in 20 N different polymers, and that the complexity of life lies in this variety.

393p loivantrinh 29-10-2023 7 3   Download

This paper examines how molecular weight affects the diffusion of molecules in the interface of laminar flow in a microfluidic channel. Two mixtures of PS with anisole and PEG were used to evaluate the effect of molecular weight on the microfluidic separation.

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The aim of this study was the determination of chromatographic profiles of major whey proteins (WP): α-lactalbumin (α-La), β-lactoglobulin A and B, (β-Lg A and B), bovine serum albumin (BSA) in dairy beverages based on Zonar milk serum. The studied WP were separated by high-performance liquid chromatography (HPLC) on Aeris XB-C18 column using gradient elution with 0.1% trifluoroacetic acid (TFA) in water and 0.1% TFA in 80% acetonitrile, at 214 nm detection.

11p lyhuyenthu 31-01-2023 2 2   Download

In the present work, the structuring and stabilising potential of flaxseed gum (FG) in whey protein isolate (WPI) cryo-hydrogels was investigated. The FG presence (0.1–1% wt.) in the heat-treated WPI dispersions (10% wt.) induced strong segregative phase separation phenomena, which were associated with a depletion flocculation mechanism.

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A proteomic workflow for a simple loss-less manual nano-fractionation (300 nL/fraction) for low μg sample amounts which avoids the need to dry down or transfer fractions to autosampler vials is shown to be feasible. It is demonstrated that the conventional procedure of drying samples down followed by reconstitution negatively affects the number of protein and peptide identifications.

10p viginny 23-12-2022 8 2   Download

Protein glycosylation is one of the most common and critical post-translational modification, which results from covalent attachment of carbohydrates to protein backbones. Glycosylation affects the physicochemical properties of proteins and potentially their function.

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Electrical asymmetrical flow field-flow fractionation (EAF4) is an interesting new analytical technique that separates proteins based on size or molecular weight and simultaneously determines the electrical characteristics of each population.

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This approach involves the use of multiple isocratic steps, together with the addition of a steep negative gradient segment (with a decrease in mobile phase strength) to "park" a slightly more retained peak somewhere along the column (at a given migration distance), while a slightly less retained compound can be eluted.

9p viginny 23-12-2022 4 3   Download

The goal of this work was to provide a technical solution for the automated optimization of multi-column systems for protein separation and fractionation. Both algorithm and a software that can be downloaded are provided.

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Plants are advantageous as biopharmaceutical manufacturing platforms because they allow the economical and scalable upstream production of proteins, including those requiring post-translational modifications, but do not support the replication of human viruses. However, downstream processing can be more labor-intensive compared to fermenter-based systems because the product is often mixed with abundant host cell proteins.

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Elucidation of protein transport mechanism in ion exchanges is essential to model separation performance. In this work we simulate intraparticle adsorption profiles during batch adsorption assuming typical process conditions for pore, solid and parallel diffusion. Artificial confocal laser scanning microscopy images are created to identify apparent differences between the different transport mechanisms.

10p viginny 23-12-2022 8 3   Download