Tissue culture medium

Activated charcoal (AC) is highly adsorbent and is often used to promote seedling growth in plant tissue culture; however, the underlying molecular mechanism remains unclear. In this study, root and leaf tissues of 10-day-old seedlings grown via immature embryo culture in the presence or absence of AC in the culture medium were subjected to global transcriptome analysis by RNA sequencing to provide insights into the effects of AC on seedling growth.

11p vihagrid 30-01-2023 6 3   Download

Different nutrient solutions alone or in combinations were applied as foliar spray on Asiatic lilium hybrid Tresor and evaluated for different characters. Plants sprayed with only water were taken as control. Other treatments were done with the dose of nutrients used in preparation of tissue culture Murashige and Skoog (MS) medium (1962) in different combinations.

7p caygaocaolon8 07-11-2020 15 2   Download

Pre-requisite for any plant biotechnological approach is development of an efficient plant regeneration system in that crop. In this regard the in vitro regeneration was achieved by using cotyledon explants from aseptically raised seedlings of popular Capsicum F1 hybrids Bharat and Indra. Seeds of both hybrids were exposed to different treatments for proper germination, were decontaminated and sown in vitro on half-strength MS medium.

13p kequaidan7 01-09-2020 11 2   Download

Hydrangea (Hortensia) is a highly popular ornamental plant for garden decoration, and now it is commercially produced for cut flower branches. The aim of this study was to set up a protocol for large scale for in vitro propagation of Hydrangea macrophylla, for this purpose two type of explant (shoot tip and node) were sterilized and the best percentage of survival explants (40%) was obtained when explants immersed for 20 min in 1.0 or 1.5 % NaOCl. For in vitro multiplication, Murashige and Skoog medium supplemented with 1.0 mg/l BA and 2.0 mg/l Kin was the best treatment on shoot number.

13p trinhthamhodang7 31-08-2020 9 1   Download

Pomegranate can be propagated by seed, air layering, grafting, shoot cuttings and tissue culture. Seed propagation is used to raise the rootstocks that can be utilized for germplasm conservation and rootstocks breeding purpose. Vegetative propagation methods are used to multiply selected genotypes that bring similar character in form of clones having similar characters to mother plant. Vegetative propagation techniques (hard wood cutting, layering) are practices to raise the saplings for commercial production in pomegranate, because it perpetuates all characteristics of each cultivar.

16p nguaconbaynhay7 15-08-2020 16 1   Download

Some glycosidic enzymes secreted by the gut of Eudrilus eugeniae and the abundance of bacteria producing similar enzymes in the gut were studied in order to know the extent of enzymatic contribution by the worm gut and the ingested bacteria. 120 different bacteria were isolated from the worm gut and their enzymatic activities were detected by using respective media and reagents. In vitro gut wall tissue culture of various gut portions was done to assess enzymatic activities in gut wall and in the culture medium.

9p angicungduoc6 20-07-2020 11 2   Download

Plant tissue culture is the method of culturing the plant cells or tissues under controlled aseptic conditions on a solid or liquid medium. It has value in basic research for the production of true to type, disease free and micro-propagated plants. Besides this also has commercial application. This review work outlines the work done on pertaining to various aspects of in vitro micro-propagation, regeneration, rooting, hardening, in potato.

7p kethamoi6 29-06-2020 24 1   Download

The experiment was conducted from September 2017 to February 2018 at Plant Tissue Culture Laboratory, Department of Fruit science, College of Horticulture and Forestry, Jhalrapatan City, Jhalawar to examine the effect of different combinations of plant growth regulators for in-vitro propagation of strawberry.

6p angicungduoc5 12-06-2020 14 0   Download

Palmvitee may act as a beneficial supplement to cartilage tissue engineering which is currently still has certain limitation on chondrocyte expansion and dedifferentiation. Our objectives in this study were to evaluate the effects of Palmvitee on the growth kinetic and phenotype gene expression of human nasal septal chondrocytes in monolayer expansion as well as cartilage reconstruction via tissue engineering technology. Human chondrocytes were cultured in mediums containing various Palmvitee concentrations.

12p 12120609 01-06-2020 15 1   Download

Plant tissue culture has been widely employed in area of agriculture, horticulture, forestry and plant breeding. It is an applied biotechnology used for mass propagation, virus elimination, secondary metabolite production and in-vitro cloning of plants. Recently, plant tissue culture has been used for the conservation of endangered plant species through short and medium term conservation also known as slow growth and cryopreservation also known as long term conservation.

9p trinhthamhodang5 16-05-2020 13 0   Download

Banana (Musa spp.) cv. Rajapuri Bale (AAB) is a popular cultivar of banana grown in Northern parts of Karnataka especially in Bagalkot, Bijapur and Belgaum. The demand for the planting material of this cultivar is high and tissue cultured plantlets is not available because of the inherent problem in the initial establishment of the culture and multiplication rate is also low.

11p trinhthamhodang5 16-05-2020 12 0   Download

Anther Culture or microspore culture is a very important and useful tool in plant breeding for efficient production of haploids and subsequent doubled haploid plants in many species, limited research has been conducted with Brassica –rapaas it is more recalcitrant in cell and tissue culture than other Brassicas. In order to access the effect of genotype on microspore embryogenesis in B –rapa, two high yielding brown Sarsoon cultivars (KS-101 and KOS-1) were studied for their response to haploid plant regeneration through Anther culture. Flower buds of 2.2 mm to 2.

4p nguaconbaynhay5 11-05-2020 9 0   Download

Liquid Murashige and Skoog (MS) medium in varying strengths, is the most commonly utilized medium for proficient rooting in the regenerated plants in doubled haploidy breeding protocols. On the other side, CPV (cocopeat: perlite: vermiculite) medium is currently being used in hardening of tissue culture regenerated plants.

9p angicungduoc4 26-04-2020 15 1   Download

Shoot apices of Physalis minima L. were cultured on fifteen full strength MS medium supplemented with various concentrations of cytokinins (BAP, KIN and TDZ). Tissue culture responses namely elongation of existing shoot and differentiation of multiple shoots were observed from the cultured explants. The establishment of cultured explants was not affected by the cytokinin concentration in the medium but overall shoot apices showed better establishment on medium supplemented with BAP than other cytokinins.

7p nguathienthan4 21-04-2020 20 1   Download

The low cost alternative components of tissue culture media were evaluated for their potential to supply nutrients and to support growing explant of Banana cultivar in vitro for their cost effectiveness against conventional high cost media. The Murashigeand Skoog (MS) salts and sucrose were replaced by vermicompost (50 g/L), table sugar (30 g/L) and coconut water (70 ml/L), and agar was replaced by filter paper, sand and wheat floor. The conventional MS medium supplemented with 30 g/L sucrose and 8 g/L agar was used as control.

7p kethamoi4 18-04-2020 13 1   Download

The present investigation was carried out at Tissue Culture Laboratory of S.K.N. Agriculture University, Jobner, India during 2018-19. Experiment was laid out using completely randomized design (CRD) with ten replications. Six culture media viz. Murashige and Skoog media, Nitsch & Nitsch media, woody plant medium, Schenk and Hildebrant medium, White’s medium and Khudson solution– C were tested for direct shoot proliferation and callus induction at most responsive level of plant growth regulators in Bael (Aegle marmelos L.).

6p caygaocaolon4 04-04-2020 12 1   Download

The present study was carried out to monitor somaclonal variations of banana cv. Ney Poovan (AB) during micropropagation using random amplified polymorphic DNA (RAPD). Sword suckers were used as explants and regenerated on MS media supplemented with BAP alone, BAP + Kinetin and TDZ at varying levels of concentrations. The regenerated shoots were again subcultured on MS medium fortified with plant growth regulators at different concentrations. Rooting was obtained on ½ MS medium supplemented with 2.00 mg/l IBA and 2 g/l of activated charcoal.

12p trinhthamhodang4 22-03-2020 15 1   Download

Capsicum annuum L. is an important vegetable crop of India and grown widely in open field as well as polyhouses. Low seed germination and higher price of seeds is major problem associate with the cultivation of this crop. To overcome these problems, plant tissue culture offers technology to generate a nursery in minimum duration with several other benefits. For this purpose, a study was designed to evaluate the effect of benzyl amino purine (BAP) on seed germination and multiplication shoot induction from shoot apex and nodal segment. Explants were obtained from in vitro grown seedlings.

7p cothumenhmong4 25-03-2020 14 1   Download

In the present investigation, a simple, rapid and efficient in vitro regeneration system was developed in green gram (Vigna radiata L. Wilczek) cv. IPM-02-03 using cotyledonary explants excised from a 4 day old seedling. Organogenic calli were developed from cotyledonary tissues within 4-6 weeks of culture, supplemented with 2.5 mg/L BAP and 1.5 mg/L NAA. Shoot regeneration was achieved from shoot tips and cotyledonary node on MS medium was supplemented with BAP 2.0 mg/l

9p cothumenhmong4 23-03-2020 18 2   Download

In this study culture mediums were tested to find a suitable commercially relevant production system for lily.

8p 12120609 18-03-2020 18 2   Download