Total DNA was extracted

This study aimed to develop a method using DNA barcode fragments to identify the hybrid Eucalyptus UP99 and UP95. The total genomic DNA was extracted from leaf samples of UP99 and UP95 and was used to amplify the DNA barcodes (matK, rbcL, trnH-psbA, ITS and ITS2) by PCR.

11p vikissinger 21-12-2023 6 1   Download

To unravel how the integrity of nuclear and mitochondrial circulating cell-free DNA (cfDNA) contributes to its plasma quantity in colorectal cancer (CRC) patients. Methods CfDNA from plasma samples of 80 CRC patients stratified by tumour stage and 50 healthy individuals were extracted. Total cfDNA concentration was determined and equal template concentrations (ETC) were analyzed by quantitative real-time PCR (qPCR) resulting in small and long fragments of KRAS, Alu and MTCO3.

14p vischultz 20-10-2023 4 1   Download

In the present investigation a total of 100 faecal samples/rectal swabs were collected from dogs suffering with gastrointestinal disorders and showing the symptoms of canine parvovirus, like bloody diarrhoea, vomiting and pyrexia. The samples were tested initially with SYBR green dye based real time PCR with different combinations of the cycles and the results obtained were further confirmed by conventional PCR. We could able to standardize the real time PCR which was more helpful in screening of the faecal samples further in large numbers.

7p trinhthamhodang9 16-12-2020 16 1   Download

Objective of this study was to identify selected species of Cordyceps genus using DNA barcodes. Seven strains of Cordyceps were collected. Total DNA extraction and purification, PCR amplification and DNA sequencing were performed with standard chemicals and kits. The candidate ITS1-5.8S-ITS2 region was amplified and sequenced. Data were analyzed using Bioedit 7.2.6 and MEGA 7 softwares. Analysis of seven obtained DNA barcode sequences of collected samples revealed that the ITS1- 5.8S-ITS2 region provided high species discriminating power for Cordyceps genus.

8p nguyenxuankha_bevandan 14-08-2020 17 1   Download

During 2015-16 survey was conducted in six different soybean growing areas of Manipur, India. The survey revealed that the maximum mean pod blight intensity was at Thoubal (21.23%) and minimum (13.82%) at Andro Research Farm, CAU. Fusarium oxysporum was isolated and identified by morphological characteristics and gene sequencing, MF512000. The pathogen was found to be the causal organism of pod blight of soybean. Total genomic DNA of the fungal cultures was extracted by using HiPurA DNA isolation Kit (HiMedia, India).

12p angicungduoc5 14-06-2020 5 0   Download

The present study was carried out for the isolation, identification and molecular characterization of Brucella species. A total of 50 samples were collected from cattle and buffalo suffering from abortions and other reproductive disorders in and around Ludhiana, Punjab. Out of the 50 samples of fetal stomach contents (25), uterine discharges (10), vaginal mucus (8) and placenta (7) processed for isolation of Brucella of which a total of four isolate were obtained and identified biochemically. All the 4 isolates were typed as biotype 1.

5p trinhthamhodang1213 29-05-2020 6 0   Download

The aim of this study was to identify the Complex Vertebral Malformation (CVM) in repeat breeder and anoestrous animals in Maharashtra state region. Total 102 animals of HF crossbred, Dangi, Gaolao and Non- descript were screened from field and cattle dairy farm. CVM is a hereditary recessive disorder in Holstein cattle which have a significant economic impact on dairy cattle breeding worldwide. Fifty repeat breeder and fifty-two anoestrous animals blood DNA were extracted by traditional method. Genotyping was performed by using PCR-RFLP analysis with the help of PstI restriction enzyme.

4p cothumenhmong5 17-05-2020 12 1   Download

Huanglongbing (HLB) or Citrus Greening Disease is a widespread and serious threat to the Citrus growing industry worldwide due to its complexity and destructiveness. On an average, the disease can cause 30 to 100 per cent yield loss around the globe depending on its severity. In Assam, an important factor associated with Citrus decline is the wide spread prevalence of Huanglongbing (HLB) in Khasi mandarin (Citrus reticulata). HLB was detected by molecular methods in Khasi mandarin orchards of Assam state in Northeastern India.

5p caygaocaolon4 04-04-2020 11 0   Download

The aim of this study was to characterize the antimicrobial peptide (bacteriocin) produced by a probiotic Lactobacillus plantarum strain DM 69 isolated from yogurt sample of Odisha. Total DNA of L. plantarum DM 69 was extracted and tested for the presence of bacteriocin genes. PCRamplified DNA showed a single band in gel which partially confirmed the presence of Plantaricin gene responsible for showing antimicrobial activity.

6p cothumenhmong4 25-03-2020 25 0   Download

Groundnut (Arachis hypogaea L.) production is constrained by a myriad of biotic and abiotic stresses which necessitate the development and use of superior varieties for increased yield. Germplasm characterisation both at the phenotypic and molecular level becomes important in all plant breeding programs. The aim of this study was to characterise groundnut genotypes at molecular level using simple sequence repeats (SSR). A total of 30 SSR markers were screened and 20 were found to be polymorphic with an average polymorphic information content (PIC) value of 0.57.

11p caygaocaolon3 09-03-2020 52 0   Download

China aster (Callistephus chinensis L. Nees.) is one of the important flower crop in India. It belongs to the family Asteraceae is native to China. Phyllody disease in China aster was first reported during 1986 from Bengaluru, India and known to be transmitted by Orosius albicinctus. The disease was characterized by chlorosis, upright growth, small leaf, short internode, stunting, profuse vegetative growth and phyllody (transformation of floral organs into leaf-like structures).

7p caygaocaolon3 27-02-2020 19 1   Download

Diamondback moth, Plutella xylostella is a major pest of cruciferous crops worldwide and it has developed resistance to almost all synthetic insecticides. It was known to harbour microorganisms which play important role in growth and development of the host. In the present study bacterial strains were isolated from third instar larvae of P. xylostella collected from Sugatur, Kolar District of the state Karnataka. Morphological and Biochemical characterization were done, among them most of the bacterial isolates were gram negative and negative for some biochemical tests.

8p cothumenhmong1 11-12-2019 20 0   Download

Significant morphological similarities within or in between species of insects has made reliable taxonomic identification difficult. DNA barcoding has appeared to be a useful tool in resolving the issues related to the identification of taxonomically difficult insect species. The Northeastern region of India is one of the mega biodiversity hotspots in the World and the climatic conditions of the region are highly conducive for reproduction and multiplication of insects.

11p cothumenhmong1 08-12-2019 12 0   Download

Recently environmental metagenomics are useful methodology to study microbial diversity in the environment as well as functional metabolic genes. This study was also based on metagenomic method to discover antibiotic resistance genes from aquatic environments. To create a metagenomic library, the environmental DNA was extracted from water and sediment sample of Thi Nghe canal, Ho Chi Minh City. Total DNA then was fragmented by sizes of 1-3 kb and inserted in to pUC19 plasmid. After transformation into E.

8p nguathienthan1 27-11-2019 15 0   Download

PCR targeting 16S rRNA gene integrated with sequence analysis were performed to investigate the prevalence and the molecular identity of Anaplasma phagocytophilum in Egyptian Rhipicephalus sanguineus ticks attached to dogs. A total of 413 adult and nymphal R. sanguineus ticks were collected while attached to 72 free-roaming dogs from four locations (Imbaba, Boulaq, Haram, Monib) in Giza Governorate, Egypt. DNA was successfully extracted from 401 specimens (133 nymphs and 268 adults). The overall prevalence rate was 13.7% and adult ticks showed a significantly higher infection rate (16.

6p kethamoi1 17-11-2019 15 0   Download

The genetic markers in inflammatory responses during mastitis afford a reasonable way for improving milk production in the Egyptian buffalo breed. Among them is the interleukin 8 Receptor Gene (IL8RB) (CXCR2); a chemokine receptor gene augments the neutrophil migration during infection. To understand its role better during mastitis in Egyptian buffalos, twenty-five dairy animals representing the normal, sub-clinically, clinically and chronically affected buffalos were randomly selected from different districts.

9p trinhthamhodang1 14-11-2019 17 2   Download

The sfp gene responsible for surfactin production was screened from the DNA extracts of 37 Bacillus spp. whose identity was confirmed by 16S rRNA gene sequence analyses. PCR screening revealed amplification of sfp gene fragments in a total of 25 isolates. Several isolates belonging to Bacillus tequilensis were found to be positive for this gene.

10p vivant2711 01-02-2019 23 1   Download

The gene 4CL1 was isolated from Chinese red pine (Pinus massoniana Lamb) and ligated into vector pPTN289 to perform transformation vector pPTN289-4CL1. This construction was transformed into Agrobacterium tumefaciens strain C58, and then transformed into Chinaberrytree (Melia azedarach L.). The transgenic Chinaberrytree was screened on selection medium (MS + 0.5mg/l 6-BA + 1mg/l vitamine B5 + 30g/l sucrose + 8g/l agar + 500mg/l Cefotaxime + 1mg/l PPT) and then extracted total DNA and tested the existence of interested gene using PCR method. ...

7p tuanlocmuido 13-12-2012 46 3   Download

While the nature and prevalence of defensive medical practices have been widely debated, most agree that the costs are exorbitant. In fact, some estimates report that the practice of defensive medicine costs the American health care system in excess of $100 billion dollars annually, which would account for up to 12% of all health care expenditures.

24p thangbienthai 17-11-2012 72 2   Download