Transform prokaryotic

More and more 3C/Hi-C experiments on prokaryotes have been published. However, most of the published modeling tools for chromosome 3D structures are targeting at eukaryotes. How to transform prokaryotic experimental chromosome interaction data into spatial structure models is an important task and in great need.

10p visilicon2711 20-08-2021 5 1   Download

Bougainvillea antiviral protein (BAP) is one among a class of the ribosomal inactivating proteins isolated from Bougainvillea spectabilis willd. Truncated version of the BAP gene was cloned and expressed in a prokaryotic vector to abolish its cytotoxicity. RNA was isolated from mature leaves of Bougainvillea and the full length cDNA was amplified by reverse transcription-PCR using template mRNA. This full length cDNA of size 756 bp was amplified using the proofreading polymerase (Q5 polymerase) and end to end gene specific primers for removal of C-terminal, the amplicon was cloned in pJET1.

9p chauchaungayxua3 07-02-2020 15 2   Download

The present study aims at evaluating the efficacy of two rapid tools, IgM dot ELISA and Latex Agglutination Test employing recombinant LipL32 as antigen in diagnosing acute leptospirosis in dogs in Kerala, a disease endemic area. One hundred and five serum samples were collected from dogs presented at Veterinary Hospitals attached to Kerala Veterinary and Animal Sciences University during the period from September 2014 to May 2016. Microscopic Agglutination test was carried out using the reference strains maintained in the Dept.

6p nguaconbaynhay1 08-12-2019 12 1   Download

Photosynthesis is the primary source of energy for all life forms (except chemolithotrophic prokaryotes). Much of the energy of photosynthesis is used to drive the synthesis of organic molecules from atmospheric CO2. How is solar energy captured and transformed into metabolically useful chemical energy? How is the chemical energy produced by photosynthesis used to create organic molecules from carbon dioxide?

48p tangtuy15 30-06-2016 25 4   Download

Alterations in the normal DNA methylation processes are known to have major consequences for embryonic development and are associated with congenital defects, autoimmunity, aging and malignant transformation. The main purpose of this book is to provide information about the importance of methylation mechanisms in human health and disease.

310p camchuong_1 04-12-2012 48 5   Download