Transgenic rice
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Ebook "Biotechnology in agriculture and forestry - Volume 59: Transgenic crops IV" deals with cereals, vegetables, root crops, herbs and spices. Section I is an introductory chapter on the impact of plant biotechnology in agriculture. Section II focuses on cereals (rice, wheat, maize, rye, pearl millet, barley, oats), while Section III is directed to vegetable crops (tomato, cucumber, eggplant, lettuce, chickpea, common beans and cowpeas, carrot, radish).
478p tachieuhoa 28-01-2024 3 2 Download
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Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency.
10p viarchimedes 26-01-2022 6 0 Download
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Physic nut (Jatropha curcas), an inedible oilseed plant, is among the most promising alternative energy sources because of its high oil content, rapid growth and extensive adaptability. Proteins encoded by MADS-box family genes are important transcription factors participated in regulating plant growth, seed development and responses to abiotic stress.
14p vijeeni2711 24-07-2021 5 0 Download
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Chilo suppressalis is a widespread rice pest that poses a major threat to food security in China. This pest can develop resistance to Cry toxins from Bacillus thuringiensis (Bt), threatening the sustainable use of insectresistant transgenic Bt rice.
19p vijeeni2711 30-06-2021 10 1 Download
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We have previously developed a rice-based oral vaccine against cholera diarrhea, MucoRice-CTB. Using Agrobacterium-mediated co-transformation, we produced the selection marker–free MucoRice-CTB line 51A, which has three copies of the cholera toxin B subunit (CTB) gene and two copies of an RNAi cassette inserted into the rice genome.
12p vilichoo2711 25-06-2021 15 1 Download
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Rice leaf blight, which is a devastating disease worldwide, is caused by the bacterium Xanthomonas oryzae pv. oryzae (Xoo). The upregulated by transcription activator-like 1 (UPT) effector box in the promoter region of the rice Xa13 gene plays a key role in Xoo pathogenicity.
10p vijichea2711 28-05-2021 14 1 Download
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As global warming becomes increasingly severe, it is urgent that we enhance the heat tolerance of crops. We previously reported that Arabidopsis thaliana PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C9 (AtPLC9) promotes heat tolerance.
11p vijichea2711 28-05-2021 9 1 Download
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Some of the pathogenic effector proteins play an active role in stimulating the plant defense system to strengthen plant resistance. In this study, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) was implemented to identify altered metabolites in transgenic rice containing over-expressed M. oryzae Systemic Defense Trigger 1 (MoSDT1) that was infected at three-time points.
12p vijichea2711 28-05-2021 7 1 Download
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Aspergillus oryzae, a safe filamentous fungus, is widely used in food and enzyme production. In this study, we examined a cultivation model using rice husks as carrier to assess the capacity of recombinant protein production in A. oryzae. The model was first tested with the A. oryzae strain expressing the DsRed reporter gene. The expression of DsRed was easily detected by the pink color of the fungal mycelium on culture media and under a fluorescence microscope. The model was then evaluated with the phyA gene encoding a phytase from the fungus Aspergillus fumigatus.
10p xilank_of 22-07-2020 19 1 Download
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Rice (Oryza sativa) productivity is adversely impacted by numerous biotic and abiotic factors. An approximate 52% of the global production of rice is lost annually owing to the damage caused by biotic factors, of which ~21% is attributed to the attack of insect pests. We have developed transgenic pyramided rice lines, endowed with enhanced resistance to major sap sucking insects, through sexual crosses made between two stable transgenic rice lines containing Allium sativum (ASAL) and Galanthus nivalis (GNA) lectin genes.
11p trinhthamhodang1213 30-05-2020 14 0 Download
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Gene upstream regularly sequences (URSs) can be used as one of the tools to annotate the biological functions of corresponding genes. In addition, tissue-preferred URSs are frequently used to drive the transgene expression exclusively in targeted tissues during plant transgenesis.
14p viminato2711 22-05-2020 12 1 Download
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PsbS is a 22-kDa Photosystem (PS) II protein involved in non-photochemical quenching (NPQ) of chlorophyll fluorescence. Rice (Oryza sativa L.) has two PsbS genes, PsbS1 and PsbS2. However, only inactivation of PsbS1, through a knockout (PsbS1-KO) or in RNAi transgenic plants, results in plants deficient in qE, the energy-dependent component of NPQ.
15p viminato2711 22-05-2020 11 0 Download
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Plants have evolved light sensing mechanisms to optimally adapt their growth and development to the ambient light environment. The COP1/SPA complex is a key negative regulator of light signaling in the well-studied dicot Arabidopsis thaliana.
15p viminato2711 22-05-2020 36 0 Download
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The insecticidal chimeric cry2AX1 gene was introduced into rice indica cultivar ASD16 with a view to impart resistance to a major lepidopteran pest, rice leaf folder. A total of 17 putative transgenic events were generated with two different constructs harbouringcry2AX1 gene and all of them were found to be positive for cry2AX1and hptII genes. The Cry2AX1 protein expression in T0transgenic rice events ranged from 0.021 to 0.093 μg/g of fresh leaf tissue.
12p trinhthamhodang5 16-05-2020 15 0 Download
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In Arabidopsis, a long day flowering plant, CONSTANS (CO) acts as a transcriptional activator of flowering under long day (LD) condition. In rice, a short day flowering plant, Hd1, the ortholog of CO, plays dual functions in respond to day-length, activates flowering in short days and represses flowering in long days.
16p vishikamaru2711 29-04-2020 12 1 Download
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Calmodulin (CaM) is an important calcium sensor protein that transduces Ca2+ signals in plant stress signaling pathways. A previous study has revealed that transgenic rice over-expressing the calmodulin gene OsCam1–1 (LOC_Os03g20370) is more tolerant to salt stress than wild type.
23p vishikamaru2711 29-04-2020 12 1 Download
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Many studies in Arabidopsis and rice have demonstrated that HD-Zip transcription factors play important roles in plant development and responses to abiotic stresses. Although common wheat (Triticum aestivum L.) is one of the most widely cultivated and consumed food crops in the world, the function of the HD-Zip proteins in wheat is still largely unknown.
23p viharuno2711 21-04-2020 7 1 Download
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Isocitrate lyase (ICL) is a key enzyme in the glyoxylate cycle. In a previous study in rice, the expression of the ICL-encoding gene (OsICL) was highly induced by salt stress and its expression was enhanced in transgenic rice lines overexpressing OsCam1–1, a calmodulin (CaM)-encoding gene.
14p viharuno2711 21-04-2020 11 1 Download
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Rice sheath blight, caused by Rhizoctonia solani is one of the most devastating diseases of rice. It is associated with significant reduction in rice productivity worldwide. A mutant variant of mannose binding Allium sativum leaf agglutinin (mASAL) was previously reported to exhibit strong antifungal activity against R. solani.
10p viuchiha2711 21-04-2020 10 1 Download
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Transgene flow through pollen and seeds leads to transgenic volunteers and feral populations in the nature, and consumer choice and economic incentives determine whether transgenic crops will be cultivated in the field.
12p viuchiha2711 21-04-2020 7 1 Download