Transwell migration

We aimed to screen novel gene signatures for ovarian cancer (OC) and explore the role of biomarkers in OC via regulating pyroptosis using bioinformatics analysis. The proliferation, migration, and invasion were determined using cell counting kit and transwell assay. Enzyme-linked immunosorbent assay was applied to measure infammatory factors. The mRNA and protein expression were detected using real-time quantitative PCR and western blot.

15p vishanshan 27-06-2024 1 1   Download

In this study, we found that LINC00852 was highly expressed in the tissue of prostate cancer using quantitative reverse transcription PCR (qRT-PCR). CCK-8 assay, colony formation experiment, Transwell migration and invasion experiments were performed to prove that the up-regulation of LINC00852 could promote the proliferation, migration and invasion of prostate cancer cells in vitro.

13p vileonardodavinci 23-12-2023 4 2   Download

There is considerable evidence that microRNAs (miRNAs) regulate several key tumor-associated genes/ pathways and may themselves have a dual regulatory function either as tumor suppressors or oncogenic miRNA, depending on the tumor type.

13p visharma 20-10-2023 4 2   Download

The present study, therefore, explored whether migration and apoptosis of peripheral blood mononuclear cells (PBMCs) and ALL blasts in high glucose conditions is regulated by A20. To this end, ALL blasts from blood samples of fifteen patients and PBMCs from healthy individuals in the absence of A20 were examined. Gene expression profile was determined by quantitative RT-PCR, cell apoptosis by flow cytometry, and cell migration by a transwell migration assay. As a result, the expression of A20 was inactivated in ALL blasts.

8p spiritedaway36 25-11-2021 10 0   Download

Long non-coding RNA (lncRNA) Wilms Tumor 1 Associated Protein Pseudogene 1 (WTAPP1) has been reported to be a critical player in the angiogenesis and migration of endothelial progenitor cells, while its involvement in cancer biology remains unknown.

6p vimaine2711 26-03-2021 13 2   Download

Emerging evidence has shown that microRNAs are involved in gastric cancer development and progression. Here we examine the role of miR-133b in gastric cancer. Methods: Quantitative real-time PCR analysis was performed in 140 patient gastric cancer tissues and 8 gastric cancer cell lines. The effects of miR-133b in gastric cancer cells metastasis were examined by scratch assay, transwell migration and matrigel invasion.

10p vijennie2711 25-09-2020 14 1   Download

The aberrant expression of microRNAs has been demonstrated to play a crucial role in the initiation and progression of hepatocarcinoma. miR-1246 expression in High invasive ability cell line than significantly higher than that in low invasive ability cell line.

11p vilisa271 22-09-2020 30 1   Download

This article focuses on the roles and mechanism of lncRNA CRNDE on the progression of HCC. MTT assay, FCM analysis, Transwell migration and invasion assay were used to detect the effects of lncRNA CRNDE on cell viability, apoptosis, migration and invasion of HCC cells.

11p viorochimaru2711 01-06-2020 5 0   Download

Meliton et al. Journal of Inflammation 2010, 7:14 http://www.journal-inflammation.com/content/7/1/14 RESEARCH Research Open Access Cytosolic group IVa phospholipase A2 mediates IL-8/CXCL8-induced transmigration of human polymorphonuclear leukocytes in vitro Angelo Y Meliton1, Nilda M Muñoz*1, Lucille N Meliton1, David C Binder1, Christopher M Osan1, Xiangdong Zhu1, Steven M Dudek1 and Alan R Leff1,2 Abstract Background: Cytosolic gIVaPLA2 is a critical enzyme in the generation of arachidonate metabolites and in induction of β2-integrin adhesion in granulocytes.

8p thulanh27 12-12-2011 49 4   Download

Migration of vascular smooth muscle cells (SMCs) from the media to intima constitutes a critical step in the development of proliferative vascular diseases. To elucidate the regulatory mechanism of vacular SMC motility, the roles of caldesmon (CaD) and its phosphorylation were investigated. Methods: We have performed Transwell migration assays, immunofluorescence microscopy, traction microscopy and cell rounding assays using A7r5 cells transfected with EGFP (control), EGFP-wtCaD or phosphomimetic CaD mutants, including EGFP-A1A2 (the two PAK sites Ser452...

12p toshiba23 18-11-2011 45 4   Download