Trypanosoma brucei

Trypanosoma brucei is the cause of the diseases known as sleeping sickness in humans (T. brucei ssp. gambiense and ssp. rhodesiense) and ngana in domestic animals (T. brucei brucei) in Africa. Procyclic trypomastigotes, the tsetse vector stage, express a surface-bound trans-sialidase that transfers sialic acid to the glycosylphosphatidylinositol anchor of procyclin, a surface glycoprotein covering the parasite surface. Trans-sialidase is a unique enzyme expressed by a few trypanosomatids that allows them to scavenge sialic acid from sialylated compounds present in the infected host. ...

10p system191 01-06-2013 44 4   Download

This study reports the identification and characterization of the regulatory subunit, TbRSU, of protein kinase A of the parasitic protozoon Trypanosoma brucei. TbRSU is coded for by a single copy gene. The protein contains an unusually long N-terminal domain, the pseudosubstrate site involved in binding and inactivation of the catalytic subunit, and two C-terminally located, closely spaced cyclic nucleotide binding domains.

10p system191 01-06-2013 36 4   Download

In this article, we report the results of an analysis of the glycolytic enzyme enolase (2-phospho-D-glycerate hydro-lase) of Trypanosoma brucei. Enolase activity was detected in both bloodstream-form and procyclic insect-stage try-panosomes, although a 4.5-fold lower specific activity was found in the cultured procyclic homogenate. Subcellular localization analysis showed that the enzyme is only pre-sent in the cytosol.

9p tumor12 20-04-2013 27 3   Download

It has been shown previously in various organisms that the peroxin PEX14 is a component of a docking complex at the peroxisomalmembrane,where it is involved in the import of matrix proteins into the organelle after their synthesis in the cytosol and recognition by a receptor. Here we present a characterization of theTrypanosoma bruceihomologue of PEX14. It is shown that the protein is associated with gly-cosomes,the peroxisome-like organelles of trypanosomatids in which most glycolytic enzymes are compartmentalized....

9p fptmusic 16-04-2013 45 2   Download

An open reading frame with fatty acid desaturase similarity was identified in the genome ofTrypanosoma brucei.The 1224 bp sequence specifies a proteinof 408 amino acids with 59% and 58% similarity toMortierella alpinaandArabid-opsis thalianaD12 desaturase, respectively, and 51% with A. thalianax3 desaturases. The histidine tracks that com-pose the iron-binding active centers of the enzyme were more similar to those of thex3 desaturases.

8p dell39 03-04-2013 45 3   Download

Cyclic nucleotide specific phosphodiesterases (PDEs) are important components of all cAMP signalling networks. In humans, 11 different PDE families have been identified to date, all of which belong to the class I PDEs. Pharmaco-logically, theyhavebecome of great interest as targets for the development of drugs for a large variety of clinical condi-tions. PDEs in parasitic protozoa have not yet been exten-sively investigated, despite their potential as antiparasitic drug targets.

11p dell39 03-04-2013 40 4   Download

Cell exposure to hypo-osmolarity and alkalinity triggers a spectrum of responses including activation of phospholipases. Glycosylphosphatidyl-inositol-specific phospholipase C (GPI-PLC) is expressed in Trypanosoma brucei, a protozoan parasite that causes human African trypanosomia-sis.

17p inspiron33 26-03-2013 50 4   Download

Papain-like cysteine proteases of pathogenic protozoa play important roles in parasite growth, differentiation and host cell invasion. The main cysteine proteases of Trypanosoma cruzi(cruzipain) and of Trypanosoma brucei (brucipain) are validated targets for the development of new chemothera-pies.

11p galaxyss3 21-03-2013 55 4   Download

6-Phosphogluconate dehydrogenase is a potential target for new drugs against African trypanosomiasis. Phosphorylated aldonic acids are strong inhibitors of 6-phosphogluconate dehydrogenase, and 4-phospho-d-erythro-nate (4PE) and 4-phospho-d-erythronohydroxamate are two of the stron-gest inhibitors of theTrypanosoma bruceienzyme.

10p media19 05-03-2013 35 3   Download

Enolase is a validated drug target inTrypanosoma brucei. To better charac-terize its properties and guide drug design efforts, we have determined six new crystal structures of the enzyme, in various ligation states and confor-mations, and have carried out complementary molecular dynamics simula-tions.

13p media19 04-03-2013 55 3   Download

African trypanosomes possess high levels of alanine aminotransferase (EC 2.6.1.2), although the function of their activity remains enigmatic, espe-cially in slender bloodstream forms where the metabolism of ketoacids does not occur. Therefore, the gene for alanine aminotransferase enzyme inTry-panosoma brucei(TbAAT) was characterized and its function assessed using a combination of RNA interference and gene knockout approaches.

13p vinaphone15 27-02-2013 55 3   Download

Nfs-like proteins have cysteine desulfurase (CysD) activity, which removes sulfur (S) from cysteine, and provides S for iron–sulfur cluster assembly and the thiolation of tRNAs. These proteins also have selenocysteine lyase activityin vitro, and cleave selenocysteine into alanine and elemental sele-nium (Se).

11p mobifone23 18-01-2013 27 3   Download

Although the protozoan parasite,Trypanosoma brucei, can acquire lipids from its environment, recent reports have shown that it is also capable of de novo synthesis of all major phospholipids. Here we provide an over-view of the biosynthetic pathways involved in phospholipid formation in T.

12p cosis54 05-01-2013 70 5   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Genetic analysis of the human infective trypanosome Trypanosoma brucei gambiense: chromosomal segregation, crossing over, and the construction of a genetic map...

0p thulanh20 12-11-2011 57 2   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Repetitive DNA is associated with centromeric domains in Trypanosoma brucei but not Trypanosoma cruzi ...

0p thulanh20 12-11-2011 46 3   Download

Mầm bệnh: Mầm bệnh của bệnh ngủ châu Phi là Trypanosoma brucei. Trong đó, ở Đông Phi chủ yếu gặp chủng T.brucei rhodesiene, còn ở Tây và Trung Phi gặp chủ yếu là T.b. gambiense. Giữa hai chủng ký sinh trùng này khó phân biệt nhau. T.b. rhodesiene thường có độc tính cao hơn, gây bệnh nặng hơn và tiến triển nhanh. T.b. gambiense thường có diễn biến mạn tính. 3.2. Dịch tễ học: 3.2.1. Nguồn bệnh: - Đối với T.b. gambiense: người là nguồn bệnh chính, vai trò của động vật có vú nuôi và thú hoang dại chưa rõ. - Đối...

6p barbieken 25-09-2010 138 14   Download