Tumor glutamate

Glioblastoma is the most common and most aggressive malignant primary brain tumor in adults. Glioblastoma cells synthesize and secrete large quantities of the excitatory neurotransmitter glutamate, driving epilepsy, neuronal death, tumor growth and invasion.

7p vishanshan 27-06-2024 2 1   Download

T cell immunoglobulin and mucin-domain containing-3 (TIM-3) is a cell surface molecule that was first discovered on T cells. However, recent studies revealed that it is also highly expressed in acute myeloid leukemia (AML) cells and it is related to AML progression. As, Glutamine appears to play a prominent role in malignant tumor progression, especially in their myeloid group, therefore, in this study we aimed to evaluate the relation between TIM-3/Galectin-9 axis and glutamine metabolism in two types of AML cell lines, HL-60 and THP-1.

15p vikoch 27-06-2024 3 1   Download

Several AA-metabolizing enzymes that are higher in GBM, are also linked to poor outcome (such as BCAT1), which makes them potential targets for therapeutic inhibition. Moreover, existing drugs that deplete asparagine and arginine may be effective against brain tumors, and should be studied in conjunction with chemotherapy.

12p vimale2711 21-08-2020 15 1   Download

The metabolic consequences of preoperative carbohydrate load in breast cancer patients are not known. The present explorative study investigated the systemic and tumor metabolic changes after preoperative per-oral carbohydrate load and their influence on tumor characteristics and survival.

23p vikuala271 13-06-2020 10 1   Download

Propionate CoA-transferase fromClostridium propionicum hasbeenpuri®edand thegene encoding the enzymehasbeen cloned and sequenced. The enzyme was rapidly and irre-versibly inactivated by sodium borohydride or hydroxyl-amine in the presence of propionyl-CoA. The reduction of the thiol ester between a catalytic site glutamate and CoA with borohydride and the cleavage by hydroxylamine were used to introduce a site-speci®c label, which was followed by MALDI-TOF-MS. This allowed the identi®cation of glutamate 324 at the active site. ...

9p research12 29-04-2013 28 3   Download

The role of the negative charge of the E139 side-chain of AnabaenaFerredoxin-NADP + reductase (FNR) in steering appropriate docking with its substrates ferredoxin, flavo-doxin and NADP + /H, that leads to efficient electron transfer (ET) is analysed by characterization of several E139 FNRmutants. Replacement of E139 affects the interaction with the different FNRsubstrates in very different ways.

10p tumor12 22-04-2013 36 1   Download

Glutamate decarboxylase is a pyridoxal 5¢-phosphate-dependent enzyme responsible for the irreversiblea-decar-boxylation of glutamate to yield 4-aminobutyrate. In Escherichia coli, as well as in other pathogenic and non-pathogenic enteric bacteria, this enzyme is a structural component of the glutamate-based acid resistance system responsible for cell survival in extremely acidic conditions (pH

8p tumor12 22-04-2013 28 1   Download

X-ray crystallography of the nonheme manganese catalase fromLactobacillus plantarum(LPC) [Barynin, V.V., Whit-taker, M.M., Antonyuk, S.V., Lamzin, V.S., Harrison, P.M., Artymiuk, P.J. & Whittaker, J.W. (2001)Structure9, 725–738] has revealed the structure of the dimanganese redox cluster together with its protein environment. The oxidized [Mn(III)Mn(III)] cluster is bridged by two solvent molecules (oxo and hydroxo, respectively) together with a l1,3bridging glutamate carboxylate and is embedded in a web of hydrogen bonds involving an outer sphere tyrosine residue (Tyr42). ...

15p tumor12 20-04-2013 25 4   Download

The X-ray structure of the ionotropic GluR2 ligand-binding core (GluR2-S1S2J) in complex with the bicyclical AMPA analogue (S)-2-amino-3-(3-hyd-roxy-7,8-dihydro-6H-cyclohepta[d]-4-isoxazolyl)propionic acid [(S)-4-AHCP] has been determined, as well as the binding pharmacology of this construct and of the full-length GluR2 receptor. (S)-4-AHCP binds with a glutamate-like binding mode and the ligand adopts two different conformations.

0p awards 06-04-2013 33 1   Download

In recent studies on heme-copper oxidases a particular glutamate residue in subunit II has been suggested to constitute the entry point of the so-called K pathway. In contrast, mutations of this residue (E78 II ) in the Paracoccus denitrificanscytochromecoxidase do not affect its catalytic activity at all (E78 II Q) or reduce it to about 50% (E78 II A); in the latter case, the mutation causes no drastic decrease in hemea3 reduction kinetics under anaerobic con-ditions, when compared to typical K pathway mutants....

9p awards 05-04-2013 26 3   Download

The initial step of tetrapyrrole biosynthesis inEscherichia coliinvolves the NADPH-dependent reduction by glutamyl-tRNA reductase (GluTR) of tRNA-bound glutamate to glutamate-1-semialdehyde. We evaluated the contribution of the glutamate moiety of glutamyl-tRNA to substrate speci-ficityin vitro using a range of substrates and enzyme variants.

6p media19 04-03-2013 31 1   Download

c-Glutamyltranspeptidase (GGT; EC 2.3.2.2), an enzyme found in organ-isms from bacteria to mammals and plants, plays a central role in glutathi-one metabolism. Structural studies of GGTs from Escherichia coliand Helicobacter pylori have revealed detailed molecular mechanisms of catalysis and maturation.

10p mobifone23 18-01-2013 45 4   Download