Ubiquitin ligase activity

UBE2O as an atypical ubiquitin-conjugating enzyme possesses an E2-E3 hybrid enzyme activity. It can regulate substrate levels or transcriptional activities by cooperating with other E3 ubiquitin ligases or forming homomeric complexes displaying intrinsic E2 and E3 activities. UBE2O controls the quality of cell proteome including protein degradation, modification, transport and location.

9p lyhuyenthu 31-01-2023 5 2   Download

Microtubule-targeting agents represent one of the most successful groups of anticancer drugs used in cancer therapy today. These drugs induce a prolonged mitotic arrest through chronic spindle assembly checkpoint (SAC) activation. Apoptosis, an outcome of the prolonged mitotic arrest, is the main mechanism by which these anticancer drugs kill cancer cells. However, not much is known about the mechanism that directs chronic SAC activation to apoptosis among other possible outcomes.

9p thiencuuchu 27-11-2021 4 1   Download

Ubiquitin ligases (E3) are the enzymes in the ubiquitin/26S proteasome pathway responsible for targeting proteins to the degradation pathway and play major roles in multiple biological activities. However, the E3 family and their functions are yet to be identified in the fruit of peach.

15p viansan2711 30-07-2021 10 1   Download

Immunomodulatory drugs (IMiDs), such as lenalidomide, are therapeutically active compounds that bind and modulate the E3 ubiquitin ligase substrate recruiter cereblon, thereby affect steady-state levels of cereblon and cereblon binding partners, such as ikaros and aiolos, and induce many cellular responses, including cytotoxicity to multiple myeloma (MM) cells.

16p vinaypyidaw2711 26-08-2020 13 2   Download

: Mantle cell lymphoma (MCL) is a B-cell hemopathy characterized by the t(11;14) translocation and the aberrant overexpression of cyclin D1. This results in an unrestrained cell proliferation.

12p vidhaka2711 31-07-2020 12 1   Download

Protein ubiquitination is a ubiquitous mechanism in eukaryotes. In Arabidopsis, ubiquitin modification is mainly mediated by two ubiquitin activating enzymes (E1s), 37 ubiquitin conjugating enzymes (E2s), and more than 1300 predicted ubiquitin ligase enzymes (E3s), of which ~470 are RING-type E3s.

15p vihashirama2711 21-05-2020 10 0   Download

SEVEN IN ABSENTIA (SINA) is a RING domain-containing ubiquitin ligase involved in Drosophila eye formation. SINA-like proteins in plants are involved in several signaling pathways.

14p vichoji2711 29-04-2020 4 1   Download

Ubiquitination is a major post-translational protein modification that regulates essentially all cellular and physiological pathways in eukaryotes. The ubiquitination process typically involves three distinct classes of enzymes, ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2) and ubiquitin ligase (E3).

20p vichoji2711 29-04-2020 8 1   Download

Post-translational modification of receptor proteins is involved in activation and de-activation of signalling systems in plants. Both ubiquitination and deubiquitination have been implicated in plant interactions with pathogens and symbionts

12p vishikamaru2711 29-04-2020 12 0   Download

As one of the main crops in the world, sterility of rice (Oryza sativa L.) significantly affects the production and leads to yield decrease. Our previous research showed that OsPUB73, which encodes U-box domain-containing protein 73, may be associated with male sterility.

13p viharuno2711 21-04-2020 5 1   Download

Murine double minute 2 (MDM2) protein exhibits many diverse biochemi-cal functions on the tumour suppressor protein p53, including transcrip-tional suppression and E3 ubiquitin ligase activity. However, more recent data have shown that MDM2 can exhibit ATP-dependent molecular chap-erone activity and directly mediate folding of the p53 tetramer. Analysing the ATP-dependent function of MDM2 will provide novel insights into the evolution and function of the protein.

12p research12 29-04-2013 54 3   Download

The suppressors of cytokine signalling (SOCS) proteins inhibit cytokine signalling by direct interaction with Janus kinases (JAKs) or activated cytokine receptors. In addition to the N-ter-minal and SH2 domains that mediate these interactions, SOCS proteins contain a C-terminal SOCS box. Evidence suggests SOCS box-containing proteins act as part of an elongin-cullin-SOCS (ECS) E3 ubiquitin ligase complex, marking target pro-teins for degradation.

35p inspiron33 26-03-2013 55 5   Download

Cbl is an adaptor protein and ubiquitin ligase that binds and is phosphory-lated by the nonreceptor tyrosine kinase Src. We previously showed that the primary interaction between Src and Cbl is mediated by the Src homology domain 3 (SH3) of Src binding to proline-rich sequences of Cbl.

15p inspiron33 23-03-2013 35 3   Download

Mutations in the E3 ubiquitin ligase parkin cause early-onset, autosomal-recessive juvenile parkinsonism (AJRP), presumably as a result of a lack of function that alters the level, activity, aggregation or localization of its sub-strates. Recently, we have reported that phospholipase Cc1 is a substrate for parkin.

12p vinaphone15 25-02-2013 38 1   Download

The nuclear–cytoplasmic shuttling protein RBCC protein interacting with protein kinase C1 (RBCK1) possesses transcriptional and ubiquitin ligase activities. We have recently reported that RBCC protein interacting with protein kinase C2 (RBCK2), a RING–in-between-RING fingers domain-lacking splice variant of RBCK1, lacks transcriptional activity, but rather represses the RBCK1-mediated transcriptional activity as a cytoplasmic tethering protein for RBCK1.

11p viettel02 20-02-2013 28 2   Download

The truncated C-terminal portion of Bid (tBid) is an important intermedi-ate in ligand-induced apoptosis. tBid has been shown to be sensitive to pro-teasomal inhibitors and downregulated by activation of the epidermal growth factor (EGF) pathway. Here, we provide evidence that tBid is a substrate of the ubiquitin ligase Itch, which can specifically interact with and ubiquitinate tBid, but not intact Bid.

12p mobifone23 18-01-2013 34 5   Download