Ubiquitylation

The eye lens is composed of fiber cells that differentiate from epithelial cells on its anterior surface. In concert with this differentiation, a set of proteins essential for lens function is synthesized, and the cellular organelles are degraded.

16p galaxyss3 19-03-2013 40 4   Download

The truncated C-terminal portion of Bid (tBid) is an important intermedi-ate in ligand-induced apoptosis. tBid has been shown to be sensitive to pro-teasomal inhibitors and downregulated by activation of the epidermal growth factor (EGF) pathway. Here, we provide evidence that tBid is a substrate of the ubiquitin ligase Itch, which can specifically interact with and ubiquitinate tBid, but not intact Bid.

12p mobifone23 18-01-2013 36 6   Download

The recognition of ubiquitylated substrates is an essential element of ubiqu-itin⁄26S proteasome-mediated proteolysis (UPP), which is mediated directly by the proteasome subunit RPN10 and⁄or RPN13, or indirectly by ubiquitin receptors containing ubiquitin-like and ubiquitin-associated domains. By pull-down and mutagenesis assays, we detected cross-species divergence of the major recognition pathways.

21p mobifone23 18-01-2013 35 4   Download