Universal primers ITS 1 and ITS 4

The present study was taken up in the Agricultural College, Bapatla during 2016-2017. Cercospora infected leaves were collected during kharif 2016 from eight different okra growing villages in Guntur district, Andhra Pradesh which were used for in planta isolation of fungal DNA and amplified using universal primers ITS 1 and ITS 4. The 550 bp amplicon thus obtained was restricted with hexa cutters, EcoRI, BamHI and tetra cutter Taq1 to find variability among Cercospora isolates.

7p gaocaolon5 14-06-2020 8 1   Download

A study was undertaken to isolate, identify and characterize the rice blast pathogen M. grisea from different rice growing regions. In the present study, ten isolates of M. grisea were categorized into different groups based on colony colour and texture. PCR was performed to identify the M. grisea isolates using the universal primers of 18S (ITS 1) and 28S (ITS 4). The PCR reaction allowed amplifying the fungal ITS fragments of 550 bp. All the isolates had the expected specific size of 550 bp which depicts molecular based confirmation of M. grisea.

9p chauchaungayxua3 07-02-2020 12 1   Download

The aim of this study was to determine the phylogenetic relationships of Trichoderma isolates obtained from NLS Tobacco region. Utilizing the sequence analysis of internal transcribed spacer-1 (ITS-1) region of the ribosomal DNA the rDNA of Five Trichoderma isolates was amplified by polymerase chain reaction (PCR) using universal primers (ITS-1 and ITS-4). PCR products were purified and these purified products were used to amplify the ITS-4 region of the five Trichoderma isolates.

8p chauchaungayxua3 07-02-2020 33 0   Download