Utilization of polymorphic SSR markers

The use of transferable cross-species/genera SSR markers is an alternative strategy to ensure availability of markers in genomic resources-limited crops such as dolichos bean. A total of 275 cross legume species/genera SSR markers were examined for their transferability to dolichos bean.

13p nguathienthan8 20-10-2020 11 1   Download

Samba Mahsuri is an elite, fine grain rice variety, which is grown extensively in India. To finger print and identify a genotype specific marker from 26 other closely related fine grain types, we utilized a 113 SSR markers distributed throughout the rice genome. Twenty six markers found to be polymorphic among the rice varieties analyzed. A twin marker combination RM 19426 +JGT 11-16.3 could unambiguously differentiate samba Mahsuri from other fine grain varieties through multiplex PCR.

7p quenchua6 15-06-2020 13 2   Download

Total 9 SSR markers were utilized to study the genetic relatedness among all thirty genotypes of turmeric. Six identified SSR markers are highly informative for genetic studies and are extremely useful in distinguishing the polymorphism rate at a specific locus in turmeric. Primer pair’s viz., 11 and 12, 5 and 6 and 13 and 14 generated higher levels of polymorphism and these could be used to differentiate turmeric genotypes under study. The molecular diversity analysis indicated presence of ample genetic diversity among the genotypes studied, which were grouped into 2 clusters.

9p angicungduoc5 14-06-2020 10 1   Download

Microsatellite markers can be used for fingerprinting of hybrids, assessing variation within parental lines and testing the genetic purity of hybrid seed lot. In this study 75 simple sequence repeat (SSR) markers well distributed on all the 10 chromosomes were employed for fingerprinting of six popular maize hybrids and their parental lines. Seven SSR markers were found to be polymorphic and produced unique fingerprints for the different hybrids. These markers can be used as referral markers for unambiguous identification and protection of the hybrids.

10p nguathienthan4 18-04-2020 17 1   Download

Oil palm (Elaeis guineensis Jacq.) having chromosome number 2n=32 and belongs to the family Arecaceae and it is a rich source of perennial vegetable-oil in India. To sustain the edible oil requirement in the country, oil palm is one of the best options due to its high oilyield (4-6 t/ha) potential compared to other annual oil-yielding crops. Polymorphic simple sequence repeat (SSR) markers play an important role in genetic diversity and mapping studies in a crop like oil palm. In the present study, total eight genotypes were screened using 110 SSR markers.

9p kethamoi4 18-04-2020 14 2   Download

The simple sequence repeat (SSR) or microsatellite marker is currently the most preferred molecular marker because of its reproducibility and codominant nature. The aim of this study was to validate SSR markers for bacterial wilt (BW) and tomato leaf curl virus disease (ToLCV) in tomato. DNA isolated from the parents Mukthi and IIHR-2195 was used to validate five SSR primers already reported for BW and ToLCV. One primer SSR20 which showed good polymorphism and reproducibility among parents were selected for further validation in F3 and F4 population.

10p caygaocaolon4 01-04-2020 11 1   Download

The exploration of genetically variable accessions is the key source of germplasm conservation and potential breeding material for the future. The more diverse group of cultivars provides an ample opportunity to breeders for re-leasing new and superior varieties, considering their quality traits for direct commercial utilization. In this study, we analyzed genetic structure, diversity and relationships in 66 accessions of Cicer arietinum using SSR molecular markers. A total of 44 PCR based SSR primers were used for molecular characterization of 66 accessions.

11p cothumenhmong4 25-03-2020 19 0   Download

Micropropagation is a highly sought after technique in the commercial production of orchid plants. This has the advantage of providing large number of plants in a short period of time. But a major constrain in the in vitro propagation technique is the somaclonal variation. It is important to produce true to type planting materials especially in case of the hybrids as they are more prone to variations. The true to type nature of the micropropagated plantlets can be confirmed by genetic fidelity analysis.

9p caygaocaolon3 27-02-2020 12 2   Download

The present investigation was to study the molecular diversity of twelve cotton genotypes, in order to select the suitable divergent parent for heterosis breeding. Totally, 55 primers were used to diversify these genotypes and 40 pairs showed clear, scorable and unambiguous bands. Out of 40, 25 primer pairs (62.55 %) were found to be polymorphic and 15 found to be monomorphic. The average number of alleles per locus was 1.8. The similarity coefficient ranged from 0.56 to 1.00 with high dissimilarity coefficient of 0.44 for the genotypes TCH 1777 with MCU 13 and TSH 0499 with SVPR 4.

7p trinhthamhodang3 17-02-2020 17 1   Download

Information on allelic differentiation is very important to elucidate the nature and extent of divergence among Brassica juncea genotypes. In present study 20 Brassica juncea L. genotypes generated 79 polymorphic alleles from 15 SSR primer pairs with an average of 5.26 alleles per locus. The polymorphism percentage was recorded to be the maximum in the case of the primer Ni4- D04 (80%) and the minimum in the case of 3 different primers namely A06-20686249, Ni3-D03 and A05-20242013 (33.3%). The polymorphism information content (PIC) values ranged from 0.41 (A01-13393871) to 0.

11p chauchaungayxua3 07-02-2020 12 0   Download