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Virus amplification
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Bệnh dịch tả lợn châu Phi (African swine fever - ASF) do virus ASF (ASFV) họ Asfarviridae gây ra, với tỷ lệ tử vong lên tới 100%. Các nghiên cứu về phương pháp LAMP (Loop-mediated isothermal amplification) trong chẩn đoán ASF hiện tại chỉ là kỹ thuật LAMP ướt không khả thi cho ứng dụng thực địa do yêu cầu điều kiện lạnh. Nghiên cứu này nhằm mục đích phát triển phương pháp LAMP khô trực tiếp hỗ trợ cho công tác chẩn đoán sớm ASF ngay tại thực địa.
9p
viellison
28-03-2024
5
1
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Part 2 book "Protocols for the diagnosis of pig viral diseases" includes content: Lateral flow assay for diagnosis of pig viral diseases; droplet digital PCR based diagnosis for porcine viral diseases; protocols for immunofluorescence techniques; polymerase spiral reaction (PSR) for the diagnosis of porcine viral diseases; recombinase polymerase amplification based diagnostics of porcine viral diseases; cell culture system for porcine virus isolation and propagation,... and other contents.
180p
muasambanhan02
18-12-2023
5
4
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Part 1 book "RT-PCR Protocols" includes content: Single cell RT-PCR on mouse embryos - A general approach for developmental biology; detection of influenza a virus neuraminidase and pb2 gene segments by one step reverse transcription polymerase chain reaction; RT-PCR amplification and cloning of large viral sequences,... and other contens.
189p
oursky05
20-09-2023
1
0
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Epizootic haemorrhagic disease virus (EHDV) and the Palyam serogroup viruses (PALV) have led to significant economic losses associated with livestock production globally. A rapid, sensitive and specific method for the detection of EHDV and PALV is critical for virus detection, monitoring, and successful control and elimination of related diseases.
11p
vigandhi
23-02-2022
12
1
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Canine distemper virus (CDV) is an enveloped negative-strand RNA virus that exhibits a high mutation rate and continuously expands the range of hosts. Notably, CDV has infected giant panda with spill over from viral reservoirs in canines.
9p
vidarwin
23-02-2022
11
1
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C. psittaci has recently emerged as an equine abortigenic pathogen causing significant losses to the Australian Thoroughbred industry, while Equine herpesvirus-1 (EHV-1) is a well-recognized abortigenic agent. Diagnosis of these agents is based on molecular assays in diagnostic laboratories.
15p
vidarwin
23-02-2022
15
1
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Porcine epidemic diarrhea virus (PEDV), an intestinal coronavirus that causes acute diarrhea and high mortality in suckling piglets, can result in high economic losses in the swine industry. In recent years, despite the use of China’s current vaccine immunization strategy, multiple types of PEDV strains were still found in immunized swine herds.
10p
vigandhi
23-02-2022
9
1
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Bunchy top disease caused by the banana bunchy top virus (BBTV) is a serious disease in hill banana. Detection of the BBTV infection in the planting material could help in the effective management of the disease. Primers were designed for the PCR amplification of BBTV coat protein (CP). In the present study, to confirm the virus infection and to quantify the viral load in the plants, samples from infected and healthy banana were subjected to PCR and southern blot hybridization.
14p
trinhthamhodang1215
23-09-2020
9
1
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Oropharyngeal Squamous Cell Carcinoma (OPSCC) is increasing in incidence despite a decline in traditional risk factors. Human Papilloma Virus (HPV), specifically subtypes 16, 18, 31 and 35, has been implicated as the high-risk etiologic agent.
10p
vibaku2711
22-07-2020
9
1
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Porcine circovirus2 (PCV2) is an emerging pathogen of porcine farming. PCV2 is a nonenveloped circular single-stranded DNA virus. PCV2 infection produces variety clinical symptoms and thus causes huge economic loss. Various diagnostic methods are available for the diagnosis of PCV2 infection. However, there is still no rapid, sensitive and practical method for detecting PCV2. Loop mediated isothermal amplification (LAMP) has been used to detect a variety of pathogenic microorganisms. In this study, we developed a LAMP for detection of PCV2.
6p
angicungduoc6
22-07-2020
20
2
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The mulberry silkworm, Bombyx mori L is a Lepidopteran insect, life cycle of which include: Egg, Larval Instars, Pupa within silky cocoon and adult moth. It is purely domesticated insect since long, which make it a quite delicate venture, easily susceptible to viral and other diseases. The viral diseases are difficult to manage due to a very short life cycle of silkworm. One of the most effective solutions is a timely detection of such infection so that to stop spread of the disease.
11p
chauchaungayxua6
26-06-2020
14
1
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Chilli (Capsicum annuum L.) is an important spice crop severely affected by the virus causes leaf curl symptoms. Efficient transmission of Chilli Leaf Curl Virus was done by using a standardized buffer for sap inoculation to chilli plants. In present investigation, we have used three simple methods i.e. inoculation by syringe, rubbing and by immersion (dip) for sap inoculation and found reproducible when the sap from the infected plants was inoculated onto healthy plants. Out of the three methods used, syringe inoculation was found most efficient method for sap transmission.
7p
kethamoi4
16-04-2020
9
2
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In the last three decades, the Baculovirus expression vector system (BEV) has evolved to one of the most widely used eukaryotic systems for heterologous protein expression including approved vaccines and therapies.
9p
vihamax2711
21-04-2020
12
1
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Ridge gourd yellow mosaic disease (RgYMD) caused by the strain of Tomato leaf curl New Delhi virus (ToLCNDV) is an emerging disease in India. Early detection of ToLCNDV in ridge gourd has great significance in the management of the disease. An innovative loop-mediated isothermal amplification (LAMP) assay was standardized for rapid detection of ToLCNDV. Assay was carried out using the set of six primers (F3, B3, FIP, BIP, LF and BF) specific to the coat protein (CP) gene of the virus.
14p
caygaocaolon3
09-03-2020
11
0
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PCR was employed to establish association of begomovirus through amplification of geminivirus specific PCR product. In order to determine the complete nucleotide sequence of DNA-A component of HgYMV, several universal primers/ abutting primers and specific primers available in the literature were tried to amplify full length DNA-A of 2.7 kb. The amplification of full length of DNA-A component of HgYMV was achieved with all the primers such as AC-abut and AV-abut, HgYMVAF and HgYMVAR and HYMVA1500F and HYMV-A1500R.
21p
nguaconbaynhay3
07-02-2020
13
1
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Nasopharyngeal carcinoma (NPC) is one of the most frequent cancer types in Vietnam, with high mortality rate. Therefore, the early diagnosis and detection of NPC is urgently needed to improve patient survival. Recent studies have confirmed that the infection of Epstein-Barr virus (EBV) and polymorphism in RPMS1 and A73 are considered as the etiological factor associated with NPC. However, in Vietnam, there are no studies relevant to the identification of polymorphism of RMPS1 and A73.
12p
chauchaungayxua1
03-12-2019
42
1
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Kỹ thuật PCR biến tính ở nhiệt độ thấp (CO - amplification at Lower Denaturation temperature - PCR; COLD - PCR) được cải tiến để ưu tiên làm giàu các đột biến (mt) chiếm tỉ lệ thấp trong quần thể thể dại (wt) qua quá trình nhân bản, nhờ đó giúp phát hiện quần thể đột biến kháng thuốc Nucleos(t)ide Analogs của HBV với độ nhạy 5% mt/wt.
11p
ketaucho
03-11-2019
48
0
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DNA tumor viruses ensure genome amplification by hijacking the cellular replication machinery and forcing infected cells to enter the S phase. The retinoblastoma (Rb) protein controls the G1⁄S checkpoint, and is targeted by several viral oncoproteins, among these the E7 protein from human papillomaviruses (HPVs).
16p
mobifone23
18-01-2013
33
2
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay
9p
dauphong15
18-02-2012
56
2
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Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Comparison of amplification enzymes for Hepatitis C Virus quasispecies analysis
11p
dauphong8
14-01-2012
44
2
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