Vitrification

During wound regeneration, both cell adhesion and adhesion-inhibitory functions must be controlled in parallel. We developed a membrane with dual surfaces by merging the properties of carboxymethyl cellulose (CMC) and collagen using vitrification.

10p viginny 30-12-2022 3 1   Download

Long term preservation of living ovarian tissues is a critical approach in human reproductive medicine as well as in the conservation of rare animal genotypes. Compared to single cell preservation, optimization of protocols for tissues is highly complex because of the diversity of cells responding differently to non-physiological conditions.

19p vijeeni2711 30-06-2021 11 0   Download

]The study was conducted to explore the cryoprotective effect of L-proline on sheep oocyte vitrification. In vitro matured sheep oocytes (n=836) were vitrified by open pulled straw method in vitrification solution consisting of 15 per cent (v/v) DMSO+ 15 per cent (v/v) EG + sucrose (0.5M) + 20 per cent FBS in TCMh (Group I) and 7.5 per cent (v/v) DMSO + 10 per cent (v/v) EG + sucrose (0.5M) + 20 per cent FBS in TCMh supplemented with 2M L-proline (Group II).

7p cothumenhmong11 11-05-2021 7 1   Download

Trong đề tài này, mục tiêu nhắm tới là những kĩ thuật động lạnh trứng bò và heo theo phương pháp mới. Nội dung cụ thể như sau: Thu và tuyển chọn trứng bò từ nguồn trứng của bò bị giết mổ tại lò mổ, đánh giá và tuyển chọn trứng heo chín từ nguồn trứng đã được IVM (In Vtro Maturation), tiến hành đông lạnh trứng thu được bằng phương pháp thủy tinh hóa trong vi giọt, đánh giá sự sống chết của trứng sau giải đông.

9p nanhankhuoctai7 01-07-2020 30 1   Download

Tissue-engineered cardiac constructs have potential in the functional recovery of heart failure; however, the preservation of these constructs is crucial for the development and widespread application of this treatment.

12p vihamax2711 21-04-2020 11 0   Download

In this study, HTO was orally administered to rats at the level of 37 kBq (1 mCi) or 370 kBq (10 mCi) to examine tissue distribution and excretion levels. After sacrifice, wet and dry tissue samples were weighed and analyzed for tissue free-water tritium (TFWT) and organically bound tritium (OBT). The mean tissue concentrations of TFWT (OBT) were 30.9 (17.8) and 4.4 (8.1).

7p minhxaminhyeu3 12-06-2019 3 0   Download

This paper presents a successful cryopreservation protocol for protocorm-like bodies (PLBs) of Dendrobium sonia-28 through the vitrification technique and the use of ascorbic acid and charcoal. Survival assessments were conducted through growth evaluations and visual and spectrophotometric 2,3,5-triphenyltetrazolium chloride assays.

10p vi4mua 24-02-2019 25 0   Download

Shoot tips of five tomato (Lycopersicon esculentum Mill.) genotypes were successfully cryopreserved by droplet vitrification. Recovered plants were studied for genetic stability by two different assays: amplified fragment length polymorphism (AFLP) using 4 primer combinations and sequencing of lycopene β-cyclase gene (LCY-B) from leaves.

11p vikimsa 22-02-2019 19 0   Download

Ascocenda Wangsa Gold is a new and fascinating orchid hybrid in the Malaysian flower industry. An efficient plant vitrification solution 2 (PVS2) cryopreservation technique was developed for protocorm-like bodies (PLBs) of Ascocenda Wangsa Gold orchid.

8p vikimsa 22-02-2019 19 0   Download

The study of the seed storage behavior and cryopreservation of embryonic axes was attempted in Citrus jambhiri genotypes using air desiccation-freezing, vitrification, and encapsulation-dehydration methods.

9p danhdanh11 09-01-2019 14 0   Download

(bq) part 2 book “biennial review of infertility” has contents: reproductive tourism, vitrification of human oocytes and embryos - an overview, popularity of icsi, clinical research design, should we eliminate fresh embryo transfer from art,… and other contents.

122p tieu_vu15 07-09-2018 19 1   Download

The area extending between Danube Canal, Danube, New and Old Danube embodies the most dynamic por- tion of Vienna’s stretch of the river. Always considered highly attractive for construction projects, the situation by the water’s edge is strongly enhanced by the inter- sections with the identity-creating urbanistic axis of Wagramer Strasse and the U1 Underground line. Thus the riverside zones are both provided with, and bordered by, signifi cant spatial fi gures and types with high recogni- tion value.

10p doidieumuonnoi 11-06-2013 56 6   Download

How shall we assess the structural change hypothesis as the basis for inner-city urban poverty? It certainly seems plausible. One has only to walk the former industrial district of any large, older city, from New York to San Francisco, to see its physical manifesta- tions. Yet some nagging problems remain.

38p doidieumuonnoi 11-06-2013 54 4   Download

building raised beds. Raised beds have soil a few inches or more higher than the surrounding area, which provides extra rooting depth, and helps the soil drain and warm up in the spring. They’re typically 3 to 4 feet wide, with mulched paths in between, so you walk on the paths and don’t compact the soil in planting areas. Raised bed sides can be made with reused lumber, broken concrete or concrete blocks, recycled plastic lum- ber, or any non-toxic material. (Don’t use treated wood.) You can also make raised beds without sides. Dig a few inches of soil out of...

12p doidieumuonnoi 11-06-2013 77 8   Download

A three gram oven-dried sample of holocellulose was placed in a 250 mL Erlenmeyer flask with a small watch glass cover. The flasks were placed into water bath that was maintained at 20 o C. The sample was then treated with 50 mL of 17.5 percent NaOH and thoroughly mixed for one minute. After the specimen was allowed to react with the solution for 29 minutes, 50 mL of distilled water was added and mixed well for another minute. The reaction continued for five more minutes. The contents of the flask were filtered by aid of...

130p loixinloi 08-05-2013 39 1   Download

A one-gram, oven-dried sample of extractive-free bamboo was placed in a 150 mL beaker. Fifteen mL of cold sulfuric acid (72 percent) was added slowly while stirring and mixed well. The reaction proceeded for two hours with frequent stirring in a water bath maintained at 20 o C. When the two hours had expired, the specimen was transferred by washing it with 560 mL of distilled water into a 1,000 mL flask, diluting the concentration of the sulfuric acid to three percent. An allihn condenser was attached to the flask. The apparatus was placed in...

78p loixinloi 08-05-2013 32 3   Download

The Trappist monks of St. Sixtus monastery in Belgium have been brewing beer since 1839. Cus- tomers must make an appointment with the monastery to buy a maximum of two 24-bottle cases per month. The scarce and highly prized beer sells for more than $15 per 11-ounce bottle. The monk’s brewing ingredients include water, malt, hops, sugar, and yeast.

0p conduongdinhmenh 07-05-2013 43 4   Download

Is this use of television vital for the community information needs? In R8069, the survey included comment on various sources or means of finding information – about important subjects. Table 6 demonstrates that TV is seen as a source of information although lower ranking in importance than face to face communication, and radio. This is not surprising given the relative ratios of television to radio. What is perhaps slightly surprising is that television as a source is used by as many people (if not slightly more) than newspapers. This may reflect literacy rates....

90p dambaotaikhoan 06-05-2013 60 4   Download

British people have seen a sample of US public broadcasting that has been so bad that it features as a snippet in humorous mainstream programmes. With the exception of the handful of RSLs the impression of the majority of British people is that community based television is so dire that no one will watch it and it has no value. In contrast to the UK, Germany has a significantly better track record, and local stations in the USA do actually form part of a comprehensive service.

17p dambaotaikhoan 06-05-2013 49 3   Download

The experience of community radio emphasises the participation and ownership of the community. There has been a strong role for local radio (private and NGO) and Pro-poor public programming. It is more than likely that there could be reflections from the different types of radio stations in the evolution of television. The private sector, if given the chance, may start a local television station – owned privately but presenting local content in local language.

133p dambaotaikhoan 06-05-2013 53 2   Download