Vitrification

This paper presents a successful cryopreservation protocol for protocorm-like bodies (PLBs) of Dendrobium sonia-28 through the vitrification technique and the use of ascorbic acid and charcoal. Survival assessments were conducted through growth evaluations and visual and spectrophotometric 2,3,5-triphenyltetrazolium chloride assays.

10p vi4mua 24-02-2019 0 0   Download

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Post-thaw development of in vitro produced buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification

4p hoami_238 19-09-2011 37 1   Download

Shoot tips of five tomato (Lycopersicon esculentum Mill.) genotypes were successfully cryopreserved by droplet vitrification. Recovered plants were studied for genetic stability by two different assays: amplified fragment length polymorphism (AFLP) using 4 primer combinations and sequencing of lycopene β-cyclase gene (LCY-B) from leaves.

11p vikimsa 22-02-2019 0 0   Download

Ascocenda Wangsa Gold is a new and fascinating orchid hybrid in the Malaysian flower industry. An efficient plant vitrification solution 2 (PVS2) cryopreservation technique was developed for protocorm-like bodies (PLBs) of Ascocenda Wangsa Gold orchid.

8p vikimsa 22-02-2019 0 0   Download

This book is dedicated to thermoset resins, an important class of polymer materials. Unlike thermoplastics, thermoset resins are characterised by a curing reaction, which converts the low molecular weight liquid resins (easy to process) into solid three- dimensional network structures. The main advantage of a thermoset over a thermoplastic is that a wide range of properties can be achieved by simply adjusting the crosslink density of the thermoset network...

424p minhtu837 11-09-2011 166 95   Download

Trứng và phôi có thể sống, tiếp tục phát triển sau khi được đông lạnh - rã đông hay không; chúng ta có thể khống chế được những rủi ro xảy ra cho trứng và phôi trong suốt quá trình thực hiện hay không là điều không dễ dàng.

16p truongthiuyen9 05-07-2011 131 19   Download

In its Ninth Programme, the English Law Commission said it would set up this joint project to examine at least two key areas of insurance contract law: non-disclosure (which will necessarily include misrepresentation) and breach of warranty. It said we would consult on whether there is a need to review other areas such as the law on insurable interest and on joint policies. No decision has yet been made as to whether other areas should be reviewed.

47p thangbienthai 23-11-2012 49 11   Download

Many beverage and fast-food marketers also engage in “community marketing,” in an effort to build relationships within the communities where they do business by giving something back to the community. McDonald’s, for example, has worked with A Better Chance Foundation to offer college preparatory school scholarships to black students in underprivileged areas. It has also developed the Black History Makers of the Future program, which highlights 30African-American children whom McDonald's feels will be among the leaders of tomorrow....

50p dangsuynghi 15-03-2013 55 9   Download

Two major approaches to cryopreservation are known, i.e., conventional freeze-thaw procedures and vitrification, which is defined as a glass-like solidification (Karlsson & Toner, 1996). While freeze-thaw procedures minimize the probability of intracellular ice formation, vitrification attempts to prevent ice formation throughout the entire sample during the cooling and warming process (Kuleshova et al., 2007). Recently, the potential of vitrification has been tested for tissue-engineered constructs.

364p kmkmkmkmkm 08-09-2012 33 7   Download

IVF and related treatments (GIFT and ZIFT, see below) are the most invasive ART treatments. Usually women try other methods first, and turn to IVF when those methods have not succeeded in pregnancy or live birth. One percent of babies in the US are born using IVF. Unlike AI, fertilization takes place outside the woman’s body in which eggs (retrieved from the woman trying to get pregnant or from an egg donor) are fertilized with sperm (from a partner or donor) in a Petri dish.

21p le_minh_nha 25-12-2012 40 7   Download

Within the overall transport process, attenuation processes may cause movement of the chemical to differ from that of the bulk flowing groundwater, for example dispersion, sorption and chemical or biological degradation of the chemical. Such attenuation processes potentially act to mitigate the impact of chemicals and are a function of both the specific chemical and geologic domain. Indeed, attenuation may vary significantly between individual chemicals and within different geological settings.

44p cao_can 29-12-2012 31 7   Download

Marketers have identified urban, low-income African-American and Latino youth as “superconsumers” of soda, candy, and snack products. Many young people report frequent snacking, unstructured meals, and eating “junk food,” such as candy, chips, and soda, for their primary meal. Recent research studying the amount and type of advertising on prime-time television programs oriented to African-American audiences compared to those for general audiences found that far more food commercials appear on shows with large...

34p dangsuynghi 15-03-2013 30 7   Download

It also reviews different valuation methods and estimated values for non-market forest goods and services in Europe. Chapter 5 gives an overview of the financing mechanisms used in the Member States and their applicability for different goods and services. It includes a theoretical review of alternative financing mechanisms for non-market forest goods and services (FG&S) as well as an empirical assessment of their use in Europe and EU Member States (EU MS).

143p dangsuynghi 15-03-2013 35 7   Download

The capital of the Fund is represented by issued and redeemable shares with no par value. The shares are entitled to distributions, if any, and to payment of a proportionate share based on the Fund’s NAV per share upon redemption. The Fund has no restrictions or specific capital requirements on the subscriptions and redemptions of shares except as disclosed in Note 8(a), if any. The relevant movements in capital are shown on the Statement of Changes in Net Assets.

6p hongphuocidol 04-04-2013 27 7   Download

The prominence of private health insurance has been buttressed by government interventions directed at PHI markets in several OECD health systems, although the effectiveness of policies aimed at increasing market size and fostering outcome quality has differed widely. Australia, Ireland, the Netherlands, Germany, Switzerland and the United States have promoted and maintained a large and viable private health insurance market because policy makers have concluded that mixed public-private coverage systems can better deliver desired health policy and social outcomes.

103p quaivatxanh 01-12-2012 28 6   Download

People often turn to egg or sperm donors when they cannot use their own eggs or sperm to become pregnant, or when they don’t have both sperm and eggs available to them, such as single women or people in LGBT relationships. They can go through a fertility clinic, an egg brokerage agency, a sperm bank, or recruit a known donor (friend, family member) or unknown donor (place Web or newspaper ad). Sperm donation can be used for AI, IVF, GIFT, and ZIFT; egg donation for all but AI. The term “donor” can be misleading as “donors” are often paid...

20p le_minh_nha 25-12-2012 31 6   Download

A synthesis of the material presented at the convening and the points made during discussion have led to the development of the recommendations for change listed above. Researchers should simultaneously pursue agendas that both inform potential intervention strategies and examine the link between food marketing and obesity. Advocates can examine current policies including the FCC and FTC regulatory environments in order to pursue policy options and advertising regulations for television and digital media. The public’s commitment is needed to accomplish these goals.

30p dangsuynghi 15-03-2013 38 6   Download

Point of view shots allow us to experience the emotions of the lead character, her anxiety and apprehension as she goes to meet the imprisoned serial killer, Hannibal Lecter, for the first time. Director Jonathan Demme discusses this scene and the influence of Alfred Hitchcock on the Silence of the Lambs in his interview with Mark Cousins as part of the BBC’s Face to Face series. This scene is also an example of how the continuity style employs over-the shoulder dialogue. In the classic continuity scene, the dialogue begins with a two-shot of the participants in the scene.

21p khanhchilam 01-04-2013 20 6   Download

building raised beds. Raised beds have soil a few inches or more higher than the surrounding area, which provides extra rooting depth, and helps the soil drain and warm up in the spring. They’re typically 3 to 4 feet wide, with mulched paths in between, so you walk on the paths and don’t compact the soil in planting areas. Raised bed sides can be made with reused lumber, broken concrete or concrete blocks, recycled plastic lum- ber, or any non-toxic material. (Don’t use treated wood.) You can also make raised beds without sides. Dig a few inches of soil out of...

12p doidieumuonnoi 11-06-2013 50 6   Download

The area extending between Danube Canal, Danube, New and Old Danube embodies the most dynamic por- tion of Vienna’s stretch of the river. Always considered highly attractive for construction projects, the situation by the water’s edge is strongly enhanced by the inter- sections with the identity-creating urbanistic axis of Wagramer Strasse and the U1 Underground line. Thus the riverside zones are both provided with, and bordered by, signifi cant spatial fi gures and types with high recogni- tion value.

10p doidieumuonnoi 11-06-2013 37 6   Download