Xylose isomerase

Xem 1-5 trên 5 kết quả Xylose isomerase

Conversion of an inactive xylose isomerase into a functional enzyme by co-expression of GroEL-GroES chaperonins in Saccharomyces cerevisiae

Second-generation ethanol production is a clean bioenergy source with potential to mitigate fossil fuel emissions. The engineering of Saccharomyces cerevisiae for xylose utilization is an essential step towards the production of this biofuel.

13p vihamax2711 21-04-2020 11 0 Download

Characterization of a novel xylose isomerase from Anoxybacillus gonensis G2T

The xylA gene encoding xylose isomerase from Anoxybacillus gonensis G2T has been cloned and successfully expressed in E. coli. Xylose isomerase was purified 10.98-fold by heat-shock and sequential column chromatography techniques to homogeneity, and the biochemical properties of the enzyme were characterized.

7p caplock2711 22-02-2019 30 1 Download

Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase

Comparative analysis of genome sequence data from mesophilic and hyperthermophilic micro-organisms has revealed a strong bias against speciﬁc thermolabile aminoacid residues (i.e. N and Q) in hyperthermophilic proteins. The N þ Q content of class II xylose isomerases (XIs) from mesophiles, moderate thermophiles, and hyperthermophiles was examined.

11p system191 01-06-2013 25 2 Download
Báo cáo Y học: Cold adaptation of xylose isomerase from Thermus thermophilus through random PCR mutagenesis Gene cloning and protein characterization

Random PCR mutagenesis was applied to theThermus thermophilus xylA gene encoding xylose isomerase. Three cold-adapted mutants were isolated with the following amino-acid substitutions: E372G, V379A (M-1021), E372G, F163L (M-1024) and E372G (M-1026). The wild-type and mutated xylAgenes were cloned and expressed in Escherichia coliHB101 using the vector pGEMÒ-T Easy, and their physicochemical and catalytic properties were determined.

7p research12 29-04-2013 51 2 Download
Báo cáo khoa học: Inﬂuence of divalent cations on the structural thermostability and thermal inactivation kinetics of class II xylose isomerases

The effects of divalent metal cations on structural thermostability and the inactivation kinetics of homologous class II d-xylose isomerases (XI; EC 5.3.1.5) from mesophilic (Escherichia coliand Bacillus licheniformis), thermophilic (Thermoanaerobacterium thermosulfurigenes), and hyperther-mophilic (Thermotoga neapolitana) bacteria were examined.

0p awards 06-04-2013 31 1 Download