Zebrafish embryos

Part 1 book "Insights from animal reproduction" includes content: Cryopreservation of sheep produced embryos – current and future perspectives, assisted reproductive techniques on south american wild mammals, the use of reproductive technologies to produce transgenic goats, major components in limiting litter size, germ cell determinant transmission, segregation, and function in the zebrafish embryo.

145p muasambanhan02 25-12-2023 7 1   Download

Trim-Away is a recent technique to rapidly deplete a protein from any cell type. Guided by antibodies, TRIM21 selects proteins for destruction. However, the applicability of this method in model organisms has not been investigated. Here, we show that Trim-Away can degrade proteins in zebrafish embryos.

6p vigalileogalilei 27-02-2022 13 1   Download

This study investigates the pesticides atrazine, chlorpyrifos, and dieldrin for endocrine disrupting, phenotypic, and neurobehavioral effects. Zebrafish embryos were exposed to various environmentally relevant concentrations of pesticides during two critical windows, 0-5 days post fertilization (dpf) throughout embryogenesis and 4- 5 dpf at early larval development.

12p thebadguys 15-01-2022 10 0   Download

In this study, we sampled 6 types of cigarettes: ThangLong, Vina, Craven, Demi, Maxx and Bastos from local stores and conducted toxicological tests on zebrafish larvae. Our results show that there are variations in toxicological properties of total particle matter collected from different cigarette brands in lethal and morphological effects on zebrafish embryos.

7p meyerowitz 25-12-2021 8 0   Download

In this study, three alkaloid fractions from Huperzia serrata (Thunb.) Trevis, Lycopodium clavatum L. and Huperzia squarrosa (G. Forst.) Trevis were tested in two toxicity models: mouse primary neuronal cell (in vitro) and zebrafish (Danio rerio) embryos (in vivo).

9p guitaracoustic02 08-12-2021 15 1   Download

Multicellular organisms require precise gene regulation during ontogeny, and epigenetic modifications, such as DNA methylation and histone modification, facilitate this precise regulation. The conservative reprogramming patterns of DNA methylation in vertebrates have been well described.

13p visilicon2711 20-08-2021 8 1   Download

Genetic studies in mouse have demonstrated the crucial function of PAX4 in pancreatic cell differentiation. This transcription factor specifies β- and δ-cell fate at the expense of α-cell identity by repressing Arx gene expression and ectopic expression of PAX4 in α-cells is sufficient to convert them into β-cells.

16p vimariana2711 22-12-2020 7 0   Download

Primary cilia are essential for Hedgehog (Hh) signal transduction in vertebrates. Although the core components of the Hh pathway are highly conserved, the dependency on cilia in Hh signaling is considered to be lower in fish than in mice, suggesting the presence of species-specific mechanisms for Hh signal transduction.

11p viwyoming2711 16-12-2020 12 1   Download

Tbx5 deficiency in zebrafish causes several abnormal phenotypes of the heart and pectoral fins. It has been reported that exogenous human growth hormone can enhance expression of downstream mediators in the growth hormone and insulin-like growth factor I (IGF-I) pathway and partially restore dysmorphogenesis in tbx5 morphants.

15p viwyoming2711 16-12-2020 13 1   Download

C-lectin family 14 Member A (Clec14a) is a transmembrane protein specifically expressed in vascular endothelial cells during embryogenesis. Previous in vitro and in vivo studies have provided conflicting data regarding Clec14a role in promoting or inhibiting angiogenesis, therefore its functional role in vascular development remains poorly understood.

10p viwyoming2711 16-12-2020 9 2   Download

Rab proteins are GTPases responsible for intracellular vesicular trafficking regulation. Rab11 proteins, members of the Rab GTPase family, are known to regulate vesicular recycling during embryonic development. In zebrafish, there are 3 rab11 paralogues, known as rab11a, rab11ba and rab11bb, sharing high identity with each other.

8p viwyoming2711 16-12-2020 13 1   Download

Zebrafish (Danio rerio) is a model organism that has emerged as a tool for cancer research, cancer being the second most common cause of death after cardiovascular disease for humans in the developed world.

12p vibaku2711 22-07-2020 12 1   Download

Developmental biology relies to a large extent on the observation and comparison of phenotypic traits through time using high resolution microscopes. In this context, transparent model organisms such as the zebrafish Danio rerio in which developing tissues and organs can be easily observed and imaged using fluorescent proteins have become very popular.

10p vihamax2711 21-04-2020 13 0   Download

Zebrafish embryos are emerging as a model for pharmacological and toxicological studies. We used zebrafish embryos to study the general toxicity and cardiovascular effects of eight methylxanthines: aminophylline, caffeine, diprophylline, doxofylline, etophylline, 3-isobutyl-1-methylxanthine (IBMX), pentoxifylline and theophylline.

12p vimax2711 30-03-2020 23 2   Download

The cardiotoxicity of isoniazid on zebrafish embryos and its underlying mechanism is unclear. Methods: Here, we exposed zebrafish embryos at 4 h post-fertilization to different levels of isoniazid and recorded the morphology and number of malformed and dead embryos under the microscope.

9p vimax2711 27-03-2020 47 2   Download

The in vitro antimicrobial potency of the bacteriocin AS-48 is well documented, but its clinical application requires investigation, as its toxicity could be different in in vitro (haemolytic and antibacterial activity in blood and cytotoxicity towards normal human cell lines) and in vivo (e.g. mice and zebrafish embryos) models. Overall, the results obtained are promising.

11p trinhthamhodang1 14-11-2019 18 1   Download

This study investigated the developmental toxicity of zinc chloride (ZnCl2) in zebrafish embryos (Brachydanio rerio, Cyprinidae, Cypriniformes). Zebrafish embryos were exposed to 5 different concentrations of ZnCl2, from the blastula stage, for 15 days under static renewal test conditions.

7p vivant2711 01-02-2019 15 2   Download

Nghiên cứu được tiến hành nhằm đánh giá tác động của ion đồng (Cu2+) ở các nồng độ 0µg/L; 500 µg/L; 1000 µg/L; 2000 µg/L lên các hoạt động sinh lí trong giai đoạn phôi của cá Ngựa vằn (Danio rerio Hamilton, 1822). Để nắm nội dung mời các bạn cùng tham khảo.

8p danhvi10 22-11-2018 28 2   Download

We have determined the structure of the fatty acid-binding protein 6 (fabp6) gene and the tissue-specific distribution of its transcripts in embryos, larvae and adult zebrafish (Danio rerio). Like most members of the vertebrate FABPmultigene family, the zebrafishfabp6 gene contains four exons separated by three introns.

10p galaxyss3 07-03-2013 34 2   Download

There are two hatching enzyme homologues in the zebrafish genome: zebrafish hatching enzymeZHE1andZHE2. Northern blot and RT-PCR analysis revealed that ZHE1 was mainly expressed in pre-hatching embryos, whereasZHE2was rarely expressed. This was consistent with the results obtained in an experiment conducted at the protein level, which demonstrated that one kind of hatching enzyme, ZHE1, was able to be purified from the hatching liquid.

13p vinaphone15 28-02-2013 52 1   Download