Ziehl neelsen stain

Tuberculosis (TB) is an ancient communicable disease caused by Mycobacterium tuberculosis. In India, RNTCP (Revised national tuberculosis control program) now known as NTEP (National tuberculosis elimination program) endorsed diagnostic steps to diagnose TB infection and disease are followed. To study the diagnostic efficacy of GeneXpert in comparison to various staining techniques in patients suspected of pulmonary tuberculosis.

7p chauchaungayxua12 29-07-2021 9 1   Download

A 2 year old male Jamunapari goat weighing 30 kilograms was presented with complaints of chronic diarrhea, progressive weight loss and poor response to therapy. Faecal sample was collected aseptically and subjected for the presence of Mycobacterium avium subspecies paratuberculosis (MAP) by Ziehl-Neelsen staining and polymerase chain reaction (PCR).

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The aim of this study is to throw a light on the importance of GeneXpert assay as a rapid and reliable test for the diagnosis of TB compared to conventional MGIT960 culture and ZN microscopy.

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This study is aimed at providing early and accurate detection of NTM from isolated mycobacterium by using routine biochemical tests. In the laboratory, specimen were processed by modified Petroff”s and cultured in a pair of Lowenstein Jensen (LJ) medium and MGIT culture, samples were processed by NALC - NaOH method, and inoculated in MGIT culture tube. The LJ medium were incubated at 37°C for 8 weeks and LJ slopes were examined daily for one weak and then every week for colonies of acid fast bacilli (AFB). Once the growth appeared, it was confirmed by Ziehl-Neelsen staining.

10p gaocaolon5 14-06-2020 9 1   Download

Tuberculosis (TB) still remains a global health problem affecting one third of world population. Early diagnosis of TB is very important for therapeutic reasons and to control the spread of infection. For developing countries with a large number of cases and financial constraints, evaluation of rapid and inexpensive diagnostic methods has a great importance.

7p trinhthamhodang1213 29-05-2020 28 0   Download

The bacteriological diagnosis of tuberculosis (TB) is largely dependent on the Ziehl-Neelsen (ZN) microscopy. This method has a low sensitivity, which can be improved by the concentration of the specimen with sodium hypochlorite or Bleach (NaOCl), followed by sedimentation after doing LED. This study was conducted to evaluate the efficiency of bleach sedimentation technique in detection of Mycobacterium tuberculosis by LED microscopy. A Total of 100 sputum samples from the patients who visited RNTCP of Rama Medical College Hospital and Research Centre between April 2015 and March 2016.

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Opportunistic infections are infections that occur due to a decrease in the immune system and can cause serious conditions in people with weak immune systems such as in people with HIV / AIDS. Cryptosporidium sp. and Giardia lamblia are examples of the opportunistic intestinal protozoa that can cause chronic diarrhea so that it becomes dehydrated and malnutrition which can increase mortality and morbidity in HIV sufferers. Observational study with cross-sectional design on 50 fecal samples of HIV / AIDS patients. Cryptosporidium sp.

7p nguaconbaynhay3 07-02-2020 18 1   Download

Screening of the patient’s family for leprosy is an important step to reduce the incidence of leprosy and also helps family members to get early treatment after skin smear test positivity. Leprosy is an endemic disease in underdeveloped or developing countries, where still a large number of cases have been reporting. Here we tried to produce the data related to leprosy in the last four years, recorded from a tertiary care hospital. A total of 619 suspected leprosy cases of both sexes were included in this study. Data related to this study was collected from laboratory registers.

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Smear microscopy and culture forms the backbone of tuberculosis (TB) laboratory investigations in tertiary healthcare facilities which have a large number of cases and financial constraints. The present study aimed to re-evaluate the efficiency of smear microscopy and culture on Lowenstein Jensen (LJ) medium for acid fast bacilli (AFB) isolated from patients with pulmonary tuberculosis. 210 samples were processed for detection of AFB by Ziehl Neelsen (ZN) staining. Concentration method of N-acetly-L-cystein-NaOH was used and the samples were isolated on LJ medium. AFB was seen in 168 (80.

6p taisaocothedung 12-01-2013 38 1   Download

Sputum samples were fi rst classifi ed according to their macroscopic aspect in the laboratory. Salivary samples were immediately removed and a new sample was requested. The time between sputum collection and analysis was recorded. A 20–30 mm smear was made on a new slide with a wooden applicator. After heat fi xation, hot Ziehl-Neelsen (ZN) staining was performed: carbol fuchsin 0.3%, slow heating until steaming, rinsing after 5 min, destaining with 25% sulfuric acid, rinsing, counterstaining with methylene blue 0.3% for 1 min, rinsing and drying.

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Mycobacterium tuberculosis không thuộc nhóm Gram + hay Gram – vì phương pháp nhuộm Gram không nhuộm được nó mặc dầu vi trùng này cũng có 1 màng bọc peptydoglycan. Ngoài màng này còn có 1 màng sáp làm cho các thuốc nhuộm Gram không thấm vào được. Phương pháp thường dùng để tìm M. tuberculosis là phết đàm bệnh nhân lên kiếng (slide smear) và nhuộm bằng Ziehl-Neelsen Stain (Acid-fast Staining Method). Thuốc nhuộm carbon-fuchsin được đổ lên smear có vi trùng rối nung sôi để chất màu có thể thấm vào vi trùng, sau đó rửa bằng...

6p thiuyen111 11-04-2011 123 18   Download