Rapid electrochemical detection of single and double nucleotide mismatches of short sequence from HIV samples
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The detection of specific DNA sequences provides the basis for detecting a wide variety of microbial and viral pathogens. Traditional methods for DNA sequencing, based on the radioisotopic and fluorescent detection, are labor and time consuming, and are, thus, not well suited for routine and rapid medical analyses, particularly for point-of-care tasks. Fast electrochemical detection of DNA sequences may greatly reduce the assay time, simplify its protocol and therefore can be used for on-site monitoring.
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