DNA replication is a fundamental part of the life cycle of all organisms. Not surprisingly many aspects of this process display profound conservation across organisms in all domains of life. The chapters in this volume outline and review the current state of knowledge on several key aspects of the DNA replication process. This is a critical process in both normal growth and development and in relation to a broad variety of pathological conditions including cancer.
DNA replication, the process of copying one double stranded DNA molecule to produce two identical copies, is at the heart of cell proliferation. This book highlights new insights into the replication process in eukaryotes, from the assembly of pre-replication complex and features of DNA replication origins, through polymerization mechanisms, to propagation of epigenetic states. It also covers cell cycle control of replication initiation and includes the latest on mechanisms of replication in prokaryotes. The association between genome replication and transcription is also addressed.
Since the discovery of the DNA structure researchers have been highly interested in the molecular basis of genome inheritance. This book covers a wide range of aspects and issues related to the field of DNA replication. The association between genome replication, repair and recombination is also addressed, as well as summaries of recent work of the replication cycles of prokaryotic and eukaryotic viruses. The reader will gain an overview of our current understanding of DNA replication and related cellular processes, and useful resources for further reading....
When you finish this chapter, you should: Understand semi-conservative mode of DNA replication; know the difference between conservative, semi-conservative and random modes of DNA replication; understand the Messelson and Stahl experiments; know that DNA replication is bi-directional and semi-discontinuous; know the functions of the three DNA polymerases in E. coli;...
Bài giảng Sự nhân đôi của DNA (DNA replication) trình bày: Sơ đồ truyền thông tin di truyền, các base (purine và pyrimidine), danh pháp, mạch đôi của DNA, cấu trúc nucleosome, vai trò của histone, sự nhân đôi NST và DNA diễn ra ở pha S và các nội dung khác.
The study of DNA advanced human knowledge in a way comparable to the major theories in physics, surpassed only by discoveries such as fire or the number zero. However, it also created conceptual shortcuts, beliefs and misunderstandings that obscure the natural phenomena, hindering its better understanding. The deep conviction that no human knowledge is perfect, but only perfectible, should function as a fair safeguard against scientific dogmatism and enable open discussion. With this aim, this book will offer to its readers 30 chapters on current trends in the field of DNA replication.
DNA replication is fundamental to cellular life on earth, and replication initiation provides the primary
point of control over this process. Replication initiation in all organisms involves the interaction of initiator
proteins with one or more origins of replication in the DNA, with subsequent regulated assembly of
two replisome complexes at each origin, melting of the DNA, and primed initiation of DNA synthesis
on leading and lagging strands.
Chapter 6 - DNA structure, replication, and recombination. This chapter includes contents: Experimental evidence for DNA as the genetic material, the Watson and Crick double helix model of DNA, genetic information in DNA base sequence, DNA replication, recombination at the DNA level.
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Initiation of bacteriophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and its relatives
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Genomic context analysis in Archaea suggests previously unrecognized links between DNA replication and translation...
The contributions of human DNA polymerases (pols)a, dande during
S-phase progression were studied in order to elaborate how these enzymes
co-ordinate their functions during nuclear DNA replication. Pol d was
three to four times more intensely UV cross-linked to nascent DNA in late
compared with early S phase, whereas the cross-linking of pols aande
remained nearly constant throughout the S phase.
During DNA replication, transcription and DNA repair in
eukaryotes, the cellular machineries performing these tasks
need to gain access to the DNA that is packaged into
chromatin in the nucleus.Chromatin is a dynamic structure
thatmodulates the access of regulatory factors to the genetic
material.A precise coordination and organization of events
in opening and closing of the chromatin is crucial to ensure
that the correct spatial and temporal epigenetic code is
maintained within the eukaryotic genome....
Replication of DNA is essential for the propagation of life. It is somewhat
surprising then that, despite the vital nature of this process, cellular organ-isms show a great deal of variety in the mechanisms that they employ to
ensure appropriate genome duplication. This diversity is manifested along
classical evolutionary lines, with distinct combinations of replicon architec-ture and replication proteins being found in the three domains of life: the
Bacteria, the Eukarya and the Archaea.
Host species specificity of the polyomaviruses simian virus
40 (SV40) and mouse polyomavirus (PyV) has been shown
to be determined by the host DNA polymerase a-primase
complex involved in the initiation of both viral and host
DNA replication. Here we demonstrate that DNA repli-cation of the related human pathogenic polyomavirus JC
virus (JCV) can be supportedin vitro by DNA polymerase
a-primase of either human or murine origin indicating that
the mechanism of its strict species specificity differs from
that of SV40 and PyV.
In this chapter, the following content will be discussed: DNA replication is semiconservative, general features of DNA replication, DNA polymerases, the mechanism of DNA replication, eukaryotic DNA replication, telomeres and telemerases, reverse transcriptase, DNA repair.