A novel core-shell-based chromatographic method supported by ratio derivative spectrophotometry for the simultaneous determination of perindopril, indapamide, and amlodipine ternary mixtures

In this work, ratio spectra of the first derivative spectrophotometric and liquid chromatographic methods have been described for the first time for the simultaneous determination of perindopril (PER), indapamide (IND), and amlodipine (AML) in dosage forms. For chromatographic separations several mobile phase compositions were tested for efficient separation with the use of a new column technology related to superficially porous particles. Optimum chromatographic separation was achieved using a Kinetex C18 column (150 × 4.6 mm I.D. 5 µm) at a flow rate of 1.5 mL min−1 . The separation was carried out at 30◦C and the diode array detector was adjusted to 215 nm.