Analysis of interactions of nitric oxide and polyamine under cadmium stress in wheat

Wheat cultivars chosen for their cadmium (Cd) tolerance (Sönmez-2001) and sensitivity (Quality) were grown in Hoagland solution for 20 days and then they were treated with 100 µM 2-(4-carboxyphenyl)-4,4,5,5-tetramethylylimidazoline-1-oxyl (cPTIO), a nitric oxide (NO) scavenger, or with 100 µM sodium nitroprusside (SNP), a NO donor, with and without 9 mM Cd. NO, free polyamines and Cd levels were analysed by using leaf and root samples taken 24 and 72 h after treatment.