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Báo cáo khoa học: 6-Phosphofructo-2-kinase and fructose-2,6-bisphosphatase in Trypanosomatidae Molecular characterization, database searches, modelling studies and evolutionary analysis

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:19

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Fructose 2,6-bisphosphate is a potent allosteric activator of trypanosomatid pyruvate kinase and thus represents an important regulator of energy meta-bolism in these protozoan parasites. A 6-phosphofructo-2-kinase, respon-sible for the synthesis of this regulator, was highly purified from the bloodstream form ofTrypanosoma bruceiand kinetically characterized. By searching trypanosomatid genome databases, four genes encoding proteins homologous to the mammalian bifunctional enzyme 6-phosphofructo-2-kinase⁄fructose-2,6-bisphosphatase (PFK-2⁄FBPase-2) were found for both T. ...

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Nội dung Text: Báo cáo khoa học: 6-Phosphofructo-2-kinase and fructose-2,6-bisphosphatase in Trypanosomatidae Molecular characterization, database searches, modelling studies and evolutionary analysis

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