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Báo cáo khoa học: 7,8-Diaminoperlargonic acid aminotransferase from Mycobacterium tuberculosis, a potential therapeutic target Characterization and inhibition studies
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Diaminopelargonic acid aminotransferase (DAPA AT), which is involved in biotin biosynthesis, catalyzes the transamination of 8-amino-7-oxonona-noic acid (KAPA) using S-adenosyl-l-methionine (AdoMet) as amino donor.Mycobacterium tuberculosisDAPA AT, a potential therapeutic tar-get, has been overproduced inEscherichia coliand purified to homogeneity using a single efficient step on a nickel-affinity column.
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