Báo cáo khoa học: A common conformationally coupled ATPase mechanism for yeast and human cytoplasmic HSP90s
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The conformationally coupled mechanism by which ATP is utilized by yeast Hsp90 is now well characterized. In contrast, ATP utilization by human Hsp90s is less well studied, and appears to operate differently. To resolve these conflicting models, we have conducted a side-by-side biochem-ical analysis in a series of mutant yeast and human Hsp90s that have been both mechanistically and structurally characterized with regard to the crys-tal structure of the yeast Hsp90 protein.
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