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Báo cáo khoa học: An engineered right-handed coiled coil domain imparts extreme thermostability to the KcsA channel

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:11

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KcsA, a potassium channel fromStreptomyces lividans, was the first ion channel to have its transmembrane domain structure determined by crystal-lography. Previously we have shown that its C-terminal cytoplasmic domain is crucial for the thermostability and the expression of the channel. Expression was almost abolished in its absence, but could be rescued by the presence of an artificial left-handed coiled coil tetramerization domain GCN4.

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Nội dung Text: Báo cáo khoa học: An engineered right-handed coiled coil domain imparts extreme thermostability to the KcsA channel

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