Báo cáo khoa học: Rotary F1-ATPase Is the C-terminus of subunit c fixed or mobile?
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F-ATP synthase synthesizes ATP at the expense of ion motive forcebyarotarycouplingmechanism. Acentral shaft, subunitc, functionally connects the ion-driven rotary motor, FO, with the rotary chemical reactor, F1.Using polarized spectrophotometry we have demonstrated previ-ously the functional rotation of the C-terminala-helical portion ofcin the supposed ‘hydrophobic bearing’ formed by the (ab)3hexagon. In apparent contradiction with these spectroscopic results, an engineered disulfide bridge between thea-helixofcand subunitadidnot impair enzyme activity....
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