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Báo cáo khoa học: An unusual amide synthetase (CouL) from the coumermycin A1 biosynthetic gene cluster from Streptomyces rishiriensis DSM 40489
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The aminocoumarin antibiotic coumermycin A1produced byStreptomyces rishiriensisDSM40489 contains two amide bonds. The biosynthetic gene cluster ofcoumermycin con-tains a putative amide synthetase gene, couL, encoding a protein of529 amino acids. CouL was overexpressed as hexahistidine fusion protein inEscherichia coliand purified by metal affinity chromatography, resulting in a nearly homogenous protein.
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