
Báo cáo khoa học: Analysis of the transcarbamoylation-dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE
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The hydrogenase maturation proteins HypF and HypE catalyze the synthesis of theCNligands of the active site iron of the NiFe-hydrogenases using carbamoylphosphate as a substrate. HypE protein fromEscherichia coliwas purified from a transformant overexpressing thehypEgene from a plasmid. PurifiedHypE in gel filtration experiments behaves predominantly as a monomer. It does not contain statisti-cally significant amounts ofmetals or of cofactors absorbing in the UV and visible light range.
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