
Báo cáo khoa học: Biochemical and molecular characterization of purified chicken pancreatic phospholipase A2
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Chicken pancreatic phospholipase A2 (ChPLA2 ) was purified from delipi-dated pancreases using ammonium sulfate and ethanol precipitation, followed by sequential column chromatography steps on MonoQ Sepha-rose and size exclusion HPLC columns. ChPLA2 was found to be a non-glycosylated monomeric protein with a molecular mass of 14 kDa and a specific activity of 400 UÆmg )1 in the presence of 1 mmsodium taur-odeoxycholate and 4 mmCaCl2with phosphatidylcholine as substrate.
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