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Báo cáo khoa học: Comparative analysis of high-affinity ligand binding and G protein coupling of the human CXCR1 chemokine receptor and of a CXCR1–Gi2a fusion protein after heterologous production in baculovirus-infected insect cells

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:13

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In order to perform biochemical and pharmacological characterization of CXCR1, we designed several CXCR1 constructs. All constructs, including a CXCR1–Gi2a fusion protein, were produced in insect cells after infection with recombinant baculovirus. The recombinant receptors exhibited specific high-affinity binding of 125 I-labelled interleukin-8, and Scatchard transformation of the binding data indicated the presence of a population of single homogenous binding sites.

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Nội dung Text: Báo cáo khoa học: Comparative analysis of high-affinity ligand binding and G protein coupling of the human CXCR1 chemokine receptor and of a CXCR1–Gi2a fusion protein after heterologous production in baculovirus-infected insect cells

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