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Báo cáo khoa học: Conformational studies of a hyperthermostable enzyme
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The structural features of the hyperthermophilic endo-b-1,3-glucanase from Pyrococcus furiosuswere studied using circular dichroism, steady-state and time-resolved fluorescence spectroscopy and anisotropy. Upon heat and chemical treatment the folded and denatured states of the protein were characterized by distinguishable spectral profiles that identified a number of conformational states.
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