Báo cáo khoa học: Critical role of His369 in the reactivity of Pseudomonas aeruginosa cytochrome cd1 nitrite reductase with oxygen
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In the denitrification pathway, Pseudomonas aeruginosacytochrome cd1 nitrite reductase catalyzes the reduction of nitrite to nitric oxide;in vitro, this enzyme is also competent in the reduction of O2 to 2H2 O. In this art-icle, we present a comparative kinetic study of the O2 reaction in the wild-type nitrite reductase and in three site-directed mutants (Tyr10fiPhe, His369fiAla and His327fiAla⁄His369fiAla) of the amino acid resi-dues close to thed1 heme on the distal side.
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