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Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control
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In haem-regulated phosphodiesterase (PDE) fromEscheri-chia coli(EcDOS), haem is bound to the PAS domain, and the redox state of the haem iron regulates catalysis by the PDEdomain.We generatedmutants ofAsp40, which forms a hydrogen bondwithHis77 (a proximal haemaxial ligand) via twowater molecules, and a salt bridge withArg85 at the protein surface. The redox potential of haem was markedly increased from67 mVvs. the standardhydrogenelectrode in the wild-type enzyme to 95 mV and 114 mV in the Ala and Asn mutants, respectively. ...
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