intTypePromotion=1
zunia.vn Tuyển sinh 2024 dành cho Gen-Z zunia.vn zunia.vn
ADSENSE

Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:6

43
lượt xem
5
download
 
  Download Vui lòng tải xuống để xem tài liệu đầy đủ

In haem-regulated phosphodiesterase (PDE) fromEscheri-chia coli(EcDOS), haem is bound to the PAS domain, and the redox state of the haem iron regulates catalysis by the PDEdomain.We generatedmutants ofAsp40, which forms a hydrogen bondwithHis77 (a proximal haemaxial ligand) via twowater molecules, and a salt bridge withArg85 at the protein surface. The redox potential of haem was markedly increased from67 mVvs. the standardhydrogenelectrode in the wild-type enzyme to 95 mV and 114 mV in the Ala and Asn mutants, respectively. ...

Chủ đề:
Lưu

Nội dung Text: Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control

ADSENSE

CÓ THỂ BẠN MUỐN DOWNLOAD

 

Đồng bộ tài khoản
2=>2