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Báo cáo khoa học: Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses
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The Arg97fiGly and Asp136fiHis mutations stabilized So-RNase HI from the psychrotrophic bacterium Shewanella oneidensis MR-1 by 5.4 and 9.7C, respectively, inTm, and 3.5 and 6.1 kJÆmol )1 , respectively, in DG(H2O). These mutations also stabilized the So-RNase HI derivative (4·-RNase HI) with quadruple thermostabilizing mutations in an additive manner.
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