Báo cáo khoa học: Direct identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS
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Intact-cell MS (ICMS) was applied for the direct detection of hydropho-bins in various species and strains ofHypocrea⁄Trichoderma.In both myce-lia and spores, dominating peaks were identified as hydrophobins by detecting mass shifts of 8 Da of reduced and unreduced forms, the analysis of knockout mutants, and comparison with protein databases.
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