
Báo cáo khoa học: Structure and function of N-acetylglucosamine kinase Identification of two active site cysteines
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N-Acetylglucosamine is a major component of com-plex carbohydrates. The mammalian salvage pathway of N-acetylglucosamine recruitment from glycoconjugate deg-radation or nutritional sources starts with phosphorylation byN-acetylglucosamine kinase. In this studywe describe the identification of two active site cysteines of the sugar kinase by site-directed mutagenesis and computer-based structure prediction.
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