Báo cáo khoa học: Direct integration of cell-free-synthesized connexin-43 into liposomes and hemichannel formation
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Proteoliposomes were directly prepared by synthesizing membrane proteins with the use of minimal protein synthesis factors isolated fromEscherichia coli(the PURE system) in the presence of liposomes. Connexin-43 (Cx43), which is a water-insoluble integral membrane protein that forms a hexa-meric complex in membranes, was cotranslationally integrated with an essentially uniform orientation in liposomes.
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