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Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulfide reductase from Pyrococcus horikoshii Implications for the disulfide metabolism of anaerobic hyperthermophiles

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:0

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We have cloned NADH oxidase homologues fromPyrococcus horikoshii and P. furiosus, and purified the recombinant form of the P. horikoshii enzyme to homogeneity from Escherichia coli. Both enzymes (previously referred to as NOX2) have been shown to act as a coenzyme A disulfide reductases (CoADR: CoA-S-S-CoA + NAD(P)H + H +fi2CoA-SH + NAD(P) + ). The P. horikoshiienzyme shows a kcat app of 7.2 s )1 with NADPH at 75C.

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Nội dung Text: Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulfide reductase from Pyrococcus horikoshii Implications for the disulfide metabolism of anaerobic hyperthermophiles

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