Báo cáo khoa học: Domain IV of mouse laminin b1 and b2 chains Structure, glycosaminoglycan modi®cation and immunochemical analysis of tissue contents
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Domain IV, consisting of about 230 residues, represents a particular protein module so far found only in lamininb1 andb2 chains. Both domains were obtained by recombi-nant production in mammalian cells. They showed a globular structure, as expected from electron microscopic examination of laminins. Fragmentb1IV was obtained as a monomer and a disul®de-bonded dimer, and both were modi®ed to50% by a single chondroitin sulfate chain attached to Ser721 of an SGD consensus sequence.
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