Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening
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The functional expression of olfactory receptors (ORs) is a primary require-ment to examine the molecular mechanisms of odorant perception and cod-ing. Functional expression of the rat I7 OR and its trafficking to the plasma membrane was achieved under optimized experimental conditions in the budding yeast Saccharomyces cerevisiae. The membrane expression of the receptor was shown by Western blotting and immunolocalization meth-ods.
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