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Báo cáo khoa học: Inhibition of pea ferredoxin–NADP(H) reductase by Zn-ferrocyanide
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Ferredoxin–NADP(H) reductases (FNRs) represent a pro-totype of enzymes involved in numerous metabolic path-ways. We found that pea FNR ferricyanide diaphorase activity was inhibited by Zn 2+ (Ki 1.57lM). Dichloro-phenolindophenol diaphorase activity was also inhibited by Zn 2+ (Ki 1.80lM), but the addition of ferrocyanide was required, indicating that the inhibitor is an arrangement of both ions.Escherichia coliFNR was also inhibited by Zn-ferrocyanide, suggesting that inhibition is a consequence of common structural features of these flavoenzymes....
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