intTypePromotion=1
zunia.vn Tuyển sinh 2024 dành cho Gen-Z zunia.vn zunia.vn
ADSENSE

Báo cáo khoa học: Investigation of the substrate specificity of a b-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:9

47
lượt xem
3
download
 
  Download Vui lòng tải xuống để xem tài liệu đầy đủ

The specificity of the Spodoptera frugiperda digestive b-glycosidase (Sfbgly50) for fucosides, glucosides and galactosides is determined by noncovalent interactions of glycone6-OHandglycone 4-OHwith the active-site residues Q39andE451.Site-directedmutagenesisandenzymesteady-state kinetics were described, showing that replacement of E451 with glutamine increased the preference of Sfbgly50 for glucosides in comparison to galactosides, whereas replacing E451 with serine had the opposite effect.

Chủ đề:
Lưu

Nội dung Text: Báo cáo khoa học: Investigation of the substrate specificity of a b-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis

ADSENSE

CÓ THỂ BẠN MUỐN DOWNLOAD

 

Đồng bộ tài khoản
4=>1