Báo cáo khoa học: Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation
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The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in the chloroplast ofChlamydomonas reinhardtiiis part of a complex that also includes phosphoribulokinase (PRK) and CP12. We identified two residues of GAPDH involved in protein–protein interactions in this complex, by changing residuesK128 andR197 intoAorE.K128A/Emutants had aKmfor NADH that was twice that of the wild type and a lower catalytic constant, whatever the cofactor.
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