intTypePromotion=1
zunia.vn Tuyển sinh 2024 dành cho Gen-Z zunia.vn zunia.vn
ADSENSE

Báo cáo khoa học: Key substrate recognition residues in the active site of a plant cytochrome P450, CYP73A1

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:12

41
lượt xem
3
download
 
  Download Vui lòng tải xuống để xem tài liệu đầy đủ

CYP73 enzymes are highly conserved cytochromes P450 in plant species that catalyse the regiospecific 4-hydroxylation ofcinnamicacid to formprecursorsofligninandmanyother phenolic compounds. A CYP73A1 homology model based on P450 experimentally solved structures was used to iden-tify active site residues likely togovern substrate binding and regio-specific catalysis. The functional significance of these residues was assessed using site-directedmutagenesis. Active site modelling predicted that N302 and I371 form a hydro-gen bond and hydrophobic contacts with the anionic site or aromatic ringofthe substrate....

Chủ đề:
Lưu

Nội dung Text: Báo cáo khoa học: Key substrate recognition residues in the active site of a plant cytochrome P450, CYP73A1

ADSENSE

CÓ THỂ BẠN MUỐN DOWNLOAD

 

Đồng bộ tài khoản
3=>0