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Báo cáo khoa học: Leishmania donovani methionine adenosyltransferase Role of cysteine residues in the recombinant enzyme
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Methionine adenosyltransferase (MAT, EC 2.5.1.6)-medi-ated synthesis ofS-adenosylmethionine (AdoMet) is a two-step process consistingof the formation of AdoMet and the subsequent cleavage of the tripolyphosphate (PPPi )mole-cule, a reaction induced, in turn, by AdoMet. The fact that the two activities, AdoMet synthesis and tripolyphosphate hydrolysis, can be measured separately is particularly useful when the site-directed mutagenesis approach is used to determine the functional role of the amino acid residues involved in each....
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