Báo cáo khoa học: Mechanism of the reaction catalyzed by dehydroascorbate reductase from spinach chloroplasts
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Dehydroascorbate reductase (DHAR) reduces dehydro-ascorbate (DHA) to ascorbate with glutathione (GSH) as the electron donor. We analyzed the reactionmechanismof spinach chloroplast DHAR, which had a much higher reaction specificity for DHA than animal enzymes, using a recombinant enzyme expressed inEscherichia coli. Kinetic analysis suggested that the reaction proceeded by a bi-uni-uni-uni-ping-pong mechanism, in which binding of DHA to the free, reduced formof the enzyme was followed bybindingofGSH....
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