intTypePromotion=1
zunia.vn Tuyển sinh 2024 dành cho Gen-Z zunia.vn zunia.vn
ADSENSE

Báo cáo khoa học: Molecular evolution of shark and other vertebrate DNases I

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:8

48
lượt xem
4
download
 
  Download Vui lòng tải xuống để xem tài liệu đầy đủ

We purified pancreatic deoxyribonuclease I (DNase I) from the shark Heterodontus japonicususing three-step column chromatography. Although its enzymatic properties resem-bled those of other vertebrateDNases I, sharkDNase I was unique inbeingabasicprotein.Full-lengthcDNAs encoding theDNases I of two shark species,H. japonicusandTriakis scyllia, were constructed from their total pancreatic RNAs using RACE. Nucleotide sequence analyses revealed two structural alterations unique to shark enzymes: substitution of twoCys residues at positions 101 and 104 (which are well conserved in all other vertebrate DNases I) and insertion of an additional Thr or Asn residue into an essential Ca 2+ -binding site. ...

Chủ đề:
Lưu

Nội dung Text: Báo cáo khoa học: Molecular evolution of shark and other vertebrate DNases I

ADSENSE

CÓ THỂ BẠN MUỐN DOWNLOAD

 

Đồng bộ tài khoản
2=>2