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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase
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Glycoside hydrolysis by retaining family 18 chitinases involves a catalytic acid (Glu) which is part of a conserved DXDXEsequence motif that spans strand four of a (ba)8 barrel (TIMbarrel) structure.Theseglycosidehydrolases are unusual in that thepositive charge emergingon theanomeric carbon after departure of the leaving group is stabilized by the substrate itself (the N-acetyl group of the distorted )1 sugar), rather than by a carboxylate group on the enzyme.
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